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Derivation and Characterization of a ES-Like Cell Line from Indian Catfish Heteropneustes fossilis Blastulas

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Title Derivation and Characterization of a ES-Like Cell Line from Indian Catfish Heteropneustes fossilis Blastulas
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Creator Anindya S. Barman, Kuldeep K. Lal,1 Gaurav Rathore,1,3 Vindhya Mohindra,1 Rajeev K. Singh,1 Akankaha Singh,1 Praveen Khare,1 and Bechan Lal4
 
Subject Not Available
 
Description Article ID 427497
A cell line designated as HFB-ES was established from blastula stage embryos of H. fossilis (Singhi). The embryonic cells were harvested and maintained in Leibovitz’s medium supplemented with 15% fetal bovine serum. The cell line had been subcultured for more than 90 passages in a period of 24 months. HFB-ES cells were able to grow at temperatures between 25 and 35°C with an optimum temperature of 28°C. The growth rate of HFB-ES was proportional to FBS concentration, with optimum growth seen at 15% FBS concentration. The originality of the cell line was confirmed by sequencing of cytochrome oxidase c subunit I (COI), cytochrome b gene, and microsatellite DNA profile. Results of chromosome complements of HFB showed normal karyo-morphology with 56 (2) diploid number of chromosomes after 40 passages which indicated that the developed cell line is chromosomally stable. The pluripotency of HFB was demonstrated by alkaline phosphatase activity and Oct-4 gene expression. Expression of GFP reporter gene was successful in HFB-ES. These results indicated that HFB-ES could be utilized for future gene expression studies.
ICAR AP Cess Fund Project
 
Date 2017-03-20T08:00:02Z
2017-03-20T08:00:02Z
2014-01-20
 
Type Research Paper
 
Identifier Anindya S. Barman, Kuldeep K. Lal, Gaurav Rathore, et al., “Derivation and Characterization of a ES-Like Cell Line from Indian Catfish Heteropneustes fossilis Blastulas,” The Scientific World Journal, vol. 2014, Article ID 427497, 9 pages, 2014. doi:10.1155/2014/427497
PMID:24574890 PMCID:PMC3918398
http://krishi.icar.gov.in/jspui/handle/123456789/3174
 
Language English
 
Relation Not Available;
 
Publisher Hindawi Publishing Corporation