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Genetic variation of striped snakehead (Channa striatus Bloch, 1793) populations using random amplified polymorphic DNA (RAPD) markers

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Title Genetic variation of striped snakehead (Channa striatus Bloch, 1793) populations using random amplified polymorphic DNA (RAPD) markers
Not Available
 
Creator Ajaz Ali Bhat
M .A .Haniffa
M. James Milton
Bilal Ahmad Paray
P. R. Divya
A. Gopalakrishnan
 
Subject Indian snakehead, Channa striatus, genetic diversity, random amplified polymorphic DNA (RAPD).
 
Description Not Available
Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was applied to analyze the genetic variation among Channa striatus collected from four geographically distant locations in India. After initial PCR screening, nine random oligodecamers viz. OPA10, OPA11, OPA15, OPAC01, OPAC03, OPAC05, OPAC07, OPAC09 and OPAC19 which generated the RAPD profile for the four C. striatus populations were selected. Amplification using these nine primers resulted in fragments ranging in length between 309-3029 bp assigned to 87 loci. Estimates of Nei’s (1978) unbiased genetic distance (D) values ranged from 0.3242-0.6320. Unweighted pair group method with arithmetic mean (UPGMA) dendrogram constructed on the basis of genetic distance revealed very close genetic relationship among C. striatus populations of river Tamirabarani (Tamil Nadu) and river Periyar (Kerala). Both populations from river Tamirabarani and Periyar were found to be genetically closer to fish populations from Kolleru Lake (Andhra Pradesh) whereas C. striatus population from Brahmani River (Orissa) was found to be genetically distant from the rest of the populations.
Department of Science Technology (DST), New Delhi (SR/SO/AS-76/2007)
 
Date 2017-03-20T08:04:16Z
2017-03-20T08:04:16Z
2014-05-28
 
Type Research Paper
 
Identifier Bhat, A. A., Haniffa, M. A., Milton, M. J., Paray, B. A., Divya, P. R., & Gopalakrishnan, A. (2014). Genetic variation of striped snakehead (Channa striatus Bloch, 1793) populations using random amplified polymorphic DNA (RAPD) markers. International Journal of Biodiversity and Conservation, 6(5), 363-372.
2141-243X
http://krishi.icar.gov.in/jspui/handle/123456789/3175
 
Language English
 
Relation Not Available;
 
Publisher Academic Journals