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Establishment and characterization of macrophage cell line from thymus of Catla catla (Hamilton, 1822)

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Title Establishment and characterization of macrophage cell line from thymus of Catla catla (Hamilton, 1822)
Not Available
 
Creator Dharmendra Kumar Chaudhary
Neeraj Sood
Gaurav Rathore
Pravata Kumar Pradhan
Peyush Punia
Naresh Kumar Agarwal
Joy Krushna Jena
 
Subject Catla catla, Cell line, Macrophage, Thymus
 
Description Not Available
A continuous cell line has been developed from
thymus explants of Catla catla and the cells have
been subcultured for 63 passages. The cells exhibited
optimum growth at 30°C in L-15 medium
containing 15% foetal bovine serum. The cultured
cells engulfed yeast cells and fluorescent latex
beads. These cells produced reactive oxygen and
nitrogen intermediates following stimulation with
lipopolysaccharide and phorbol esters. The culture
supernatant from the cultured cells had lysozyme
activity and these cells demonstrated Fc receptors.
Almost all the cells were positive for alpha-naphthyl
acetate esterase enzyme suggesting that the
cells are of macrophage lineage and therefore, the
cell line was designated as catla thymus macrophage
(CTM) cell line. CTM cells formed aggregates
around zoospores of Aphanomyces invadans, but
were unable to inhibit the germination of spores.
The karyotype analysis of CTM cells at 25th passage
revealed a typical diploid model with 50
chromosomes per cell. Partial amplification,
sequencing and alignment of fragments of two
mitochondrial genes 16S rRNA and cytochrome c
oxidase subunit 1 confirmed that the CTM cell line
originated from C. catla. This cell line should be
useful for studying the role of macrophages in differentiation
and maturation of thymocytes and
can be a source of macrophage-specific enzymes
and cytokines
Not Available
 
Date 2017-03-20T11:56:21Z
2017-03-20T11:56:21Z
2014-01
 
Type Research Paper
 
Identifier Chaudhary, D. K., Sood, N., Rathore, G., Pradhan, P. K., Punia, P., Agarwal, N. K., & Jena, J. K. (2014). Establishment and characterization of macrophage cell line from thymus of Catla catla (Hamilton, 1822). Aquaculture Research, 45(2), 299-311
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/3190
 
Language English
 
Relation Not Available;
 
Publisher John Wiley & Sons, Inc.