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Karyological and molecular diversity in three freshwater species of the genus Channa (Teleostei, Perciformes) from India.

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Title Karyological and molecular diversity in three freshwater species of the genus Channa (Teleostei, Perciformes) from India.
Not Available
 
Creator Ravindra Kumar
Basdeo Kushwaha
Naresh S. Nagpure
Bijoy K. Behera
Wazir S. Lakra
 
Subject Channa spp
cytogenetics
Manipur
nucleolar organizer region
rDNA
 
Description Not Available
Genetic variability was assessed among three species of genus Channa, namely C. gachua, C. punctata and C. striata, using karyological tools including karyomorphology, silver nitrate and chromomycin A3 staining of nucleolar organizer regions (NORs) and C-banding. Additionally, sequence variation in partial 18S and divergent domain 1 of 28S ribosomal DNA was utilized for inter-species diversity assessment. Diploid chromosome numbers of 32, 40 and 52 in the three species were observed and the karyotype formula was derived as 18m + 12sm + 2st (fundamental arm number [FN] = 62); 6m + 2sm + 10st + 22t (FN = 48) and 12m + 10sm + 14st + 16t (FN = 86) in C. punctata, C. striata and C. gachua, respectively. Similarly, variations were also observed in number and location of silver and chromomycin A3 stained NORs and C-banding patterns. DNA sequencing generated sizes for 18S of 243, 248, 245 bp and for 28S of 401, 392 and 393 bp in C. gachua, C. punctata and C. striata, respectively. The results indicated that the cytogenetic and molecular markers are useful for the assessment of genetic diversity among the fish species and could provide valuable information for germplasm evaluation and ex situ conservation.
Not Available
 
Date 2017-07-15T13:00:24Z
2017-07-15T13:00:24Z
2013
 
Type Research Paper
 
Identifier Ravindra Kumar , Basdeo Kushwaha , Naresh S. Nagpure , Bijoy K. Behera & Wazir S. Lakra (2013) Karyological and molecular diversity in three freshwater species of the genus Channa (Teleostei, Perciformes) from India, Caryologia, 66:2, 109-119, DOI: 10.1080/00087114.2013.821829
0008-7114 (Print)
2165-5391 (Online)
http://krishi.icar.gov.in/jspui/handle/123456789/4685
 
Language English
 
Relation Not Available;
 
Publisher Taylor & Francis Group