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DNA bar-Coding of Indian coldwater fishes of genus Schizothorax (Family: Cyprinidae) from western Himalaya

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Title DNA bar-Coding of Indian coldwater fishes of genus Schizothorax (Family: Cyprinidae) from western Himalaya
Not Available
 
Creator Suresh Chandra
Ashoktaru Barat
Mahender Singh
Birender Kumar Singh
Rakesh Matura
 
Subject Cytochrome Oxidase
Subunit I %
Bar-coding %
Fish %
MtDNA %
Schizothorax
 
Description Not Available
The rapid and accurate characterization of species using morphological data is a critical constraint.
To overcome this, species identification using molecular tools has been supplemented in many studies in
present era. The present study was designed to test the utility of Cytochrome Oxidase I (COI) DNA barcodes
for the identification of two commercially important coldwater species of Genus Schizothorax (Snow trout),
Family Cyprinidae, from Uttarakhand Himalayas. COI gene (655 bp) was amplified using PCR and sequenced.
The mean intra-specific nucleotide sequence divergence was 1.75% (range 0.00-3.50%) and inter-specific
divergence of S. richardsonii is 0.00% (range 0.00040-0.00080%) and S. progastus is 0.00% (range 0.00036-
0.000206), respectively. Although, DNA bar-coding aims to develop species identification systems, some
phylogenetic signal was apparent in the data. It was concluded that COI sequencing or ‘bar-coding’, was found
to be suitable for the identification of coldwater fish species.
Indian Council of Agriculture Research (ICAR) and Department of Biotechnology (DBT), New Delhi
 
Date 2017-07-18T10:59:02Z
2017-07-18T10:59:02Z
2012
 
Type Research Paper
 
Identifier Chandra, S., Barat, A., Singh, M., Singh, B.K. and Matura, R., 2012. DNA bar-coding of Indian coldwater fishes of genus Schizothorax (family: Cyprinidae) from Western Himalaya. World Journal of Fish and Marine Sciences, 4(4), pp.430-435.
2078-4589
http://krishi.icar.gov.in/jspui/handle/123456789/4754
 
Language English
 
Relation Not Available;
 
Publisher IDOSI Publications