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Production and characterization of a monoclonal antibody against putative T lymphocytes of Catla catla

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Title Production and characterization of a monoclonal antibody against putative T lymphocytes of Catla catla
Not Available
 
Creator Dharmendra K. Chaudhary
Neeraj Sood
P. K. Pradhan
N. K. Agarwal
Gaurav Rathore
 
Subject Catla catla
cELISA
Flow cytometry
Immunoperoxidase test
Monoclonal antibody
Thymus
T lymphocytes
Western blotting
 
Description Not Available
Catla catla is the fastest growing Indian major carp and one of the major aquaculture species in South Asia. A monoclonal antibody (MAb) designated B8 MAb was produced against nylon wool-enriched thymus mononuclear cells of C. catla. This MAb did not show reactivity with macrophage and epithelial cell lines derived from catla thymus in cellular ELISA. In flow cytometric analysis of gated lymphocytes, the percentage of B8 positive (B8+) cells in thymus (n = 10, 500–600 g) was determined to be 77.7 %. Similarly, the percentage of B8+ cells in kidney, spleen and blood (n = 5) was 15.08, 1.1 and 32.17 %, respectively. Western blotting of reduced membrane proteins showed that B8 MAb reacted with a polypeptide having a molecular weight of 168.2 kDa. In indirect immunoperoxidase test, B8+ cells appeared to be lymphoid cells with a high nucleus to cytoplasmic ratio. B8 reactive cells were densely packed in central region of thymus whereas, a few cells were found to be positive in kidney and spleen sections. B8 MAb also reacted with a significant population of lymphocytes in blood smears. Considering the economic importance of C. catla, this MAb should be a useful tool for studying immune response of this fish species.
Not Available
 
Date 2017-07-18T11:06:28Z
2017-07-18T11:06:28Z
2012-09
 
Type Research Paper
 
Identifier Chaudhary, D.K., Sood, N., Pradhan, P.K., Agarwal, N.K. and Rathore, G., 2012. Production and characterization of a monoclonal antibody against putative T lymphocytes of Catla catla. In Vitro Cellular & Developmental Biology-Animal, 48(8), pp.483-492.
http://krishi.icar.gov.in/jspui/handle/123456789/4756
 
Language English
 
Relation Not Available;
 
Publisher SPRINGER LINK