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Molecular identification and codon optimization analysis of major virulence encoding genes of Aeromonas hydrophila

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Title Molecular identification and codon optimization analysis of major virulence encoding genes of Aeromonas hydrophila
Not Available
 
Creator Vijai Singh
Dharmendra Kumar Chaudhary
Indra Mani
Pallavi Somvanshi
Gaurav Rathore
Neeraj Sood
 
Subject Aeromonas hydrophila
virulence factor
PCR
codon optimization
vaccine
 
Description Not Available
Aeromonas hydrophila is an opportunistic pathogen of aquatic and terrestrial animals and human
beings. The aim of this study is to investigate 25 isolates of A. hydrophila from 40 fish muscles and 12
water samples. All isolates are screened for the presence of virulence factors such as aerolysin,
hemolysin and lipase both phenotypically as well as genotypically. All the isolates produced lipase,
whereas only 60% of isolates produced β-hemolysis with RBCs. The presence of 3 virulence genes
aerolysin, hemolysin and lipase were confirmed by PCR assay, which gave positive PCR amplification
for conserved fragment. These were also codon optimized for over expression in Escherichia coli and
obtained the high codon adaptation index in comparison to wild type of DNA sequences. These finding
may help to better expression of gene for scale up production of vaccine candidates.
Not Available
 
Date 2017-07-25T09:47:45Z
2017-07-25T09:47:45Z
2010-05-18
 
Type Research Paper
 
Identifier Singh, V., Chaudhary, D.K., Mani, I., Somvanshi, P., Rathore, G. and Sood, N., 2010. Molecular identification and codon optimization analysis of major virulence encoding genes of Aeromonas hydrophila. African Journal of Microbiology Research, 4(10), pp.952-957.
1996-0808
http://krishi.icar.gov.in/jspui/handle/123456789/4985
 
Language English
 
Relation Not Available;
 
Publisher ResearchGate