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Development and characterization of a new epithelial cell line PSF from caudal fin of Green chromide, Etroplus suratensis (Bloch, 1790)

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Title Development and characterization of a new epithelial cell line PSF from caudal fin of Green chromide, Etroplus suratensis (Bloch, 1790)
Not Available
 
Creator T. Raja Swaminathan
Wazir S. Lakra
A. Gopalakrishnan
V. S. Basheer
B. Khushwaha
K. A. Sajeela
 
Subject Green chromide
Pearlspot
Etroplus suratensis
PSF cell line
 
Description Not Available
A new cell line [pearlspot fin (PSF)] has been developed from caudal fin of Etroplus suratensis, a brackish/freshwater fish cultivated in India. The cell line was maintained in Leibovitz's L-15 supplemented with 10% fetal bovine serum (FBS). The PSF cell line consisted predominantly of epithelial-like cells. The cells were able to grow at temperatures between 25 degrees C and 32 degrees C with optimum temperature of 28 degrees C. The growth rate of PSF cells increased as the FBS proportion increased from 2% to 20% at 28 degrees C with optimum growth at the concentration of 10% FBS. One marine fish virus (fish nodavirus) was tested on this cell line and found not susceptible. After confluency, the cells were subcultured with a split ratio of 1:2. The cells showed epithelial-like morphology and reached confluency on the third d after subculture. Polymerase chain reaction amplification of mitochondrial 16S rRNA and COI indicated identity of this cell line with those reported from this fish species, confirming that the cell line was of pearlspot origin. The cells were successfully cryopreserved and revived at the tenth, 25th, and 35th passages. The bacterial extracellular products from Vibrio cholerae MTCC 3904 were found to be toxic to PSF. Karyotyping analysis indicated that the modal chromosome number was 48.
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Date 2017-07-25T10:26:38Z
2017-07-25T10:26:38Z
2010-09
 
Type Research Paper
 
Identifier Swaminathan TR, Lakra WS, Gopalakrishnan A, Basheer VS, Khushwaha B, Sajeela KA. Development and characterization of a new epithelial cell line PSF from caudal fin of Green chromide, Etroplus suratensis (Bloch, 1790). In Vitro Cell Dev Biol Anim. 2010 Sep;46(8):647-56. doi: 10.1007/s11626-010-9326-y. Epub 2010 Jun 25. PubMed PMID: 20577829.
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http://krishi.icar.gov.in/jspui/handle/123456789/4988
 
Language English
 
Relation Not Available;
 
Publisher SPRINGER