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Development of primary culture from gills of Tor tor (Hamiltonbuchanan)

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Title Development of primary culture from gills of Tor tor (Hamiltonbuchanan)
Not Available
 
Creator Kamalendra
Smita Kapoor
J. Sharma
M. Goswami
G. Rathore
W.S.Lakra
 
Subject Fish culture
Gills
Aquaculture
Management
Production
 
Description Not Available
Primary culture of gill cells was initiated from gill filament explants of game fish, Tor tor. The explants were cultured in Leibovitz L-15 medium with 5, 10 and 20% foetal bovine serum (FBS). The attachment efficiency of cells was serum-dependent, while increased FBS concentration did not stimulate further proliferation of cells. First radiation of typical gill epithelial cells was observed after 38 h, of attachment of explants. There was high proliferation for the first 7 days then a stable plateau till 10 days, followed by a decline phase from 14 th day. Following removal of cells, the explants produced further cell outgrowth which was especially active at the proliferation phase (10 days). Removal of these cells caused the explants to produce a further proliferation of cells reaching confluency in 4รข 10 days. The use of gill explants to establish cell culture system of fish has advantages which include longevity of the culture and successive proliferations from explants, which could provide a useful tool for the investigation of toxicological and diagnostic studies.
Not Available
 
Date 2017-07-24T07:14:19Z
2017-07-24T07:14:19Z
1001
 
Type Research Paper
 
Identifier Kapoor, S., Sharma, J., Goswami, M., Rathore, G. and Lakra, W.S., 2011. Development of primary culture from gills of Tor tor (Hamilton-buchanan). Indian Journal of Animal Sciences (India).
0367-8318
http://krishi.icar.gov.in/jspui/handle/123456789/4947
 
Language English
 
Relation Not Available;
 
Publisher INTERNATIONAL INFORMATION SYSTEM FOR THE AGRICULTURAL SCIENCE AND TECHNOLOGY