Record Details

Molecular characterization, structure prediction and insilico analysis of hydrocarbon degrading surfactin synthetase from marine sponge-associated Bacillus licheniformis NIOT-06

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Molecular characterization, structure prediction and insilico analysis of hydrocarbon degrading surfactin synthetase from marine sponge-associated Bacillus licheniformis NIOT-06
Not Available
 
Creator Anburajan, L.
Meena, B.
Rangamaran, V.R
Toms C. Joseph
Vinithkumar, N.V.
Gopa, D
Kirubagaran, R
 
Subject Marine sponge
Bacillus licheniforms NIOT-06
Biosurfactant gene
Surfactin synthetase
Recombinant biosurfactant
 
Description Not Available
An open reading frame encoding the surfactin synthetase gene (srfA) was cloned from the Bacillus licheniformis NIOT-06 genome. An expression plasmid containing the srfA operon was introduced into Escherichia coli M15 cells and the recombinant biosurfactant was purified and quantified. Purified recombinant surfactin revealed excellent emulsification activity. HPLC fraction of the purified recombinant surfactin revealed significant surface tension reducing property. Secondary structure of the surfactin synthetase enzyme was predicted to have alpha-helical structure. Three dimensional structure model of surfactin synthase was found to be 0.243 Å units.
Not Available
 
Date 2018-12-02T09:55:17Z
2018-12-02T09:55:17Z
2016-12
 
Type Research Paper
 
Identifier Anburajan, L., Meena, B., Rangamaran, V.R., Toms C. Joseph, Vinithkumar, N.V., Gopa, D. and Kirubagaran, R. (2016) - Molecular characterization, structure prediction and in silico analysis of hydrocarbon degrading surfactin synthetase from marine sponge-associated Bacillus licheniformis. Gene Reports, 5: 40-44.
2452-0144
http://krishi.icar.gov.in/jspui/handle/123456789/15021
 
Language English
 
Relation Not Available;
 
Publisher Elsevier