Molecular characterization, structure prediction and insilico analysis of hydrocarbon degrading surfactin synthetase from marine sponge-associated Bacillus licheniformis NIOT-06
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Title |
Molecular characterization, structure prediction and insilico analysis of hydrocarbon degrading surfactin synthetase from marine sponge-associated Bacillus licheniformis NIOT-06
Not Available |
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Creator |
Anburajan, L.
Meena, B. Rangamaran, V.R Toms C. Joseph Vinithkumar, N.V. Gopa, D Kirubagaran, R |
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Subject |
Marine sponge
Bacillus licheniforms NIOT-06 Biosurfactant gene Surfactin synthetase Recombinant biosurfactant |
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Description |
Not Available
An open reading frame encoding the surfactin synthetase gene (srfA) was cloned from the Bacillus licheniformis NIOT-06 genome. An expression plasmid containing the srfA operon was introduced into Escherichia coli M15 cells and the recombinant biosurfactant was purified and quantified. Purified recombinant surfactin revealed excellent emulsification activity. HPLC fraction of the purified recombinant surfactin revealed significant surface tension reducing property. Secondary structure of the surfactin synthetase enzyme was predicted to have alpha-helical structure. Three dimensional structure model of surfactin synthase was found to be 0.243 Å units. Not Available |
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Date |
2018-12-02T09:55:17Z
2018-12-02T09:55:17Z 2016-12 |
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Type |
Research Paper
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Identifier |
Anburajan, L., Meena, B., Rangamaran, V.R., Toms C. Joseph, Vinithkumar, N.V., Gopa, D. and Kirubagaran, R. (2016) - Molecular characterization, structure prediction and in silico analysis of hydrocarbon degrading surfactin synthetase from marine sponge-associated Bacillus licheniformis. Gene Reports, 5: 40-44.
2452-0144 http://krishi.icar.gov.in/jspui/handle/123456789/15021 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier
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