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Measurement of antimicrobial activity of crustacean haemolymph against aquatic pathogenic bacteria and serum protein profiling

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Title Measurement of antimicrobial activity of crustacean haemolymph against aquatic pathogenic bacteria and serum protein profiling
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Creator Haripriya, B.H.
Nadella, R.K.
Muthulakshmi, T.
Greeshma, S.S.
Prasad, M.M.
 
Subject Not Available
 
Description Not Available
Crustacean haemolymph carries several antimicrobial proteins that are needed for battling with the external pathogens. These proteins serve non-specific immune functions such as nodule formation, phagocytosis and
encapsulation of bacterial pathogens. The present study is carried out to determine the antimicrobial activity of crustacean haemolymph against commonly occurring aquatic pathogens. The reference bacterial cultures used in this study are: Pseudomonas aeruginosa and Aeromonas hydrophila. Haemolymph was aseptically drawn from Litopenaeus vannamei and Scylla serrata using 23 gauge needle. Sterile Brain-heart infusion (BHI) broth was prepared and the freshly grown bacterial cultures were inoculated with supplementation of 1 per cent haemolymph. Incubation was carried out at 30 degree celsius for 48 hours with measuring the bacterial
growth using spectrophotometer at 4, 8, 16, 24 and 48 h for determining antibacterial activity. The results obtained from the above study were compared with readings of two controls. The crustacean haemolymph
had shown significant effect on the growth of pathogens which is evidenced from the decrease in optical density in haemolymph supplemented broth for crab and shrimp respectively for A. hydrophila and P. aeruginosa. The probable reason may be lysis of bacterial cells by the antimicrobial proteins present in haemolymph. Crustacean serum protein profiling was carried out using SDSPAGE to detect antimicrobial proteins. In SDS-PAGE, the antibacterial proteins found in shrimp haemolymph were 20, 25, 40 and 60 kDa, whereas in crab haemolymph they were 20 and 25 kDa. In conclusion, crustacean haemolymph has the ability to clear the external pathogens from the haemolymph.
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Date 2019-11-25T07:52:38Z
2019-11-25T07:52:38Z
2017-11-21
 
Type Other
 
Identifier Haripriya, B.H.,Nadella, R.K.,Muthulakshmi, T.,Greeshma, S.S. and Prasad, M.M. (2017) Measurement of antimicrobial activity of crustacean haemolymph against aquatic pathogenic bacteria and serum protein profiling. In: (Thomas, S.N., Rao, B.M., Madhu, V.R., Asha, K.K., Binsi, P.K., Viji, P., Sajesh, V.K. and Jha, P.N., Eds.) Fostering Innovations in Fisheries and Aquaculture: Focus on Sustainability and Safety – Book of Abstracts, 11th Indian Fisheries and Aquaculture Forum, ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch, 21-24 November, 2017, Kochi, India, pp. 313-314.
978-81-933623-1-0
http://krishi.icar.gov.in/jspui/handle/123456789/25259
 
Language English
 
Relation Not Available;
 
Publisher ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch