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Incidence of Aspergillus fungal species in cured fish of Gujarat based on internal transcribed spacer regions 1 and 4

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Title Incidence of Aspergillus fungal species in cured fish of Gujarat based on internal transcribed spacer regions 1 and 4
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Creator Sivaraman, G.K.
Vishnuvinayagam, S.
Jha, A.K.
Remya, S.
Renuka, V.
Ajeesh, K.
Vanik, D.
Prasad, M.M.
 
Subject Not Available
 
Description Not Available
Fungal infestations cause quality losses and often pose serious health hazards to the consumers. The sun dried fish samples such as ribbon fish, bombay duck, catfish, horse mackerel, dhoma, leather jacket, prawn and acetes were collected from the dried fish processing facilities and commercial markets from Veraval and
Nawabhandar in Gujarat. Ten gram of the fish samples were homogenised in sterile normal saline and serial dilution was made from 10-1 to 10-6 and spread onto a duplicate plate of Rose Bengal Chloramphenicol Agar
and incubated at 25 to 30 degree celsius for 5 days. The total fungal counts ranged from 2.0 x 101 to
5.0 x 102 cfu. per gram and the higher counts were frequently seen in the ribbon fish sample. The morphologically distinct colony was streaked on to Sabouraud Dextrose Agar for DNA isolation. The fungal DNA was isolated and purified as per the manufacturer’s guidelines and PCR was carried out with the universal primers for the 18 S gene with the ITS1 and ITS4 regions of 670 bp. The DNA sequences were submitted to the European Nucletide Archives and found that Aspergillus nidulans, Aspergillus oryzae, Aspergillus flavus, Aspergillus sydowii, A. foetidus and Aspergillus rugulosa. The highest similarity was observed between Aspergillus
flavus with Aspergillus oryzae and Aspergillus nidulans with Aspergillus rugulosa. The least genetic similarity was found between Aspergillus flavus with Aspergillus rugulosa and Aspergillus oryzae with Aspergillus rugulosa. The phylogenetic tree revealed that two clade were formed among the fungal species with one clade
belongs to Aspergillus flavus, Aspergillus rugulosa and Aspergillus sydowii and the another with Aspergillus flavus and Aspergillus oryzae. The present study shows the presence of different pathogenic fungal species which can be from different sources of contamination at various stages of dry fish processing.
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Date 2019-11-25T08:41:19Z
2019-11-25T08:41:19Z
2017-11-21
 
Type Other
 
Identifier Sivaraman, G.K.,Vishnuvinayagam, S.,Jha, A.K.,Remya, S.,Renuka, V.,Ajeesh, K.,Vanik, D. and Prasad, M.M. (2017) Incidence of Aspergillus fungal species in cured fish of Gujarat based on internal transcribed spacer regions 1 and 4. In: (Thomas, S.N., Rao, B.M., Madhu, V.R., Asha, K.K., Binsi, P.K., Viji, P., Sajesh, V.K. and Jha, P.N., Eds.) Fostering Innovations in Fisheries and Aquaculture: Focus on Sustainability and Safety – Book of Abstracts, 11th Indian Fisheries and Aquaculture Forum, ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch, 21-24 November, 2017, Kochi, India, pp. 390-391.
978-81-933623-1-0
http://krishi.icar.gov.in/jspui/handle/123456789/25330
 
Language English
 
Relation Not Available;
 
Publisher ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch