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Development of PCR-RFLP technique for the identification of Sardinella gibbosa from various processed fishery products

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Title Development of PCR-RFLP technique for the identification of Sardinella gibbosa from various processed fishery products
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Creator Rehana Raj
Jeyasekaran, G.
Jeyashakila, R.
 
Subject Not Available
 
Description Not Available
Sardinella gibbosa caught from Tuticorin coast of Tamil Nadu was identified from other species of sardines viz. Sardinella longiceps, S. albella, S. fimbriata, and S. sirm by employing PCR-RFLP technique. 3 lots of
samples, the raw sardine along with cooked and frozen samples were prepared out of these five samples of sardines. Cooked product was prepared by cooking the sardine samples at 100 degree celsius for 15 min. Frozen samples were prepared by freezing at minus 40 degree celsius for 24h in ultra-freezer. DNA was extracted from control and processed products followed by the amplification of mitochondrial cytochrome b gene by using specific primer C-CB285Df and C-CB431R. Mitochondrial gene was amplified at 147bp
irrespective for all the sardine species for both control and treatment groups. Amplified gene was subjected to restriction digestion by using two enzymes HinfI and MnlI. The digested DNA samples were then run on
10 per cent Polyacrylamide Gel Electrophoresis (PAGE) to view the band pattern. The digested band pattern showed similarity in the case of S. gibbosa, which yielded 107 bp and was the unique band pattern for both
control and processed samples. Other sardine species yielded almost similar band pattern which was not able to differentiate easily. Hence S. gibbosa was identified from other species of sardines, which is one of the
major species landed in Tuticorin coast, having a good market demand and export value. So there is a chance of mixing S. gibbosa meat with other low value sardine varieties. Hence PCR-RFLP can be recommended as an effective technique and can be used for routine species identification even from the processed fish products.
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Date 2019-11-25T08:43:54Z
2019-11-25T08:43:54Z
2017-11-21
 
Type Other
 
Identifier Rehana Raj,Jeyasekaran, G. and Jeyashakila, R. (2017) Development of PCR-RFLP technique for the identification of Sardinella gibbosa from various processed fishery products. In: (Thomas, S.N., Rao, B.M., Madhu, V.R., Asha, K.K., Binsi, P.K., Viji, P., Sajesh, V.K. and Jha, P.N., Eds.) Fostering Innovations in Fisheries and Aquaculture: Focus on Sustainability and Safety – Book of Abstracts, 11th Indian Fisheries and Aquaculture Forum, ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch, 21-24 November, 2017, Kochi, India, pp. 405-406.
978-81-933623-1-0
http://krishi.icar.gov.in/jspui/handle/123456789/25348
 
Language English
 
Relation Not Available;
 
Publisher ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch