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Isolation of crude fucoidan from Sargassum wightii using conventional and ultra-sonication extraction methods

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Title Isolation of crude fucoidan from Sargassum wightii using conventional and ultra-sonication extraction methods
Not Available
 
Creator Mandakini Devi, H.
Anuj Kumar
Tejpal, C.S.
Elavarasan, K.
Pankaj Kishore
Ashok Kumar, K.
 
Subject Seaweed
Fucoidan
Ultra-sonication
Hot water
Antioxidant
 
Description Not Available
Fucoidan, a fucose rich sulphated polysaccharide, generally found in cell wall matrix of brown seaweeds possess diverse physiological properties. Crude fucoidan was extracted from Sargassum wightii, a brown seaweed common along the Indian coastline. A comparison was made between hot water extraction (HWE) and ultra-sonication extraction (UAE) methods. The UAE method gave higher yield than HWE method. The UAE fucoidan had lighter colour than HWE, as indicated by Hunter colour values. The water activity values for fucoidans obtained were 0.14 for HWE and 0.19 for UAE. The sulphate content was similar in both fucoidans .However, HWE fucoidan had higher content of fucose. The Fourier transform infrared spectroscopy spectra confrmed the presence of sulphate groups in both the fucoidans. The absorption bands at 1245.37 per centimeter and 1294.99 per centimeter in the HWE and UAE fucoidan respectively, indicated the S–O stretching due to sulphate groups in the fucose monomer. The thermal transition temperature as obtained by differential scanning calorimetry for HWE was lower than UAE fucoidan. HWE fucoidan had higher DPPH
radical scavenging and reducing power than UAE fucoidan. On the other hand, UAE fucoidan had higher metal
chelating activity. The present study demonstrated that ultra-sonication can be an effective alternative for the
extraction of sulphated polysaccharide from seaweed, in term of higher yield. The health benefts of Sargassum
wightii and fucoidan obtained from it, could be explored by incorporation in different food systems.
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Date 2019-11-22T04:33:12Z
2019-11-22T04:33:12Z
2019-10
 
Type Research Paper
 
Identifier Mandakini Devi, H.,Anuj Kumar,Tejpal, C.S.,Elavarasan, K.,Pankaj Kishore and Ashok Kumar, K. (2019) Isolation of crude fucoidan from Sargassum wightii using conventional and ultra-sonication extraction methods. Bioactive Carbohydrates and Dietary Fibre. 20: 100200
2212-6198
http://krishi.icar.gov.in/jspui/handle/123456789/24934
 
Language English
 
Relation Not Available;
 
Publisher Elsevier