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Inhibitory effect of marine algae collected from the East and West coast of India against luciferase and luminescence producing Vibrio harveyi

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Title Inhibitory effect of marine algae collected from the East and West coast of India against luciferase and luminescence producing Vibrio harveyi
Not Available
 
Creator Krishnamoorthy Sivakumar
S. Kannappan
 
Subject Algae extracts, antagonism, Vibrio harveyi, anti-luciferase, anti-luminescence.
 
Description Not Available
This study explored the development of inhibitors from marine algae against luminescence disease causing Vibrio harveyi. Organic solvents such as ethyl acetate (EA) and methanol:chloroform (CM) were treated to extract compounds from marine algae such as Skeletonema costatum, Ulva fasciata and Kappaphycus alvarezii. Antagonistic effect of algae extracts was determined against marine V. harveyi. EA extract of S. costatum and U. fasciata at 300 µg showed maximum zone of inhibition (12.6 and 12.3 mm) against V. harveyi. Similarly, CM extract of S. costatum also showed maximum zone of inhibition (8.3 mm). When crude EA extract of S. costatum at 300 µg/ml was used for V. harveyi, the level of luciferase was reduced to three to seven counts per second (CPS) as compared to the control for 4 days as read by luminometer. 300 g/ml of CM extract of U. fasciata, reduced the luciferase to 3 to 6 CPS. When EA extract of S. costatum at (300 µg/ml) was treated against V. harveyi, the luminescence was reduced 10 to 15 CPS. The CM extract from U. fasciata showed reduction in the luminescence production (7 to 13 CPS). Considering the cumulative effect of anti-luminescence and anti-Vibrio activities, 300 g of crude extract prepared from S. costatum in both solvents were confirmed as inhibitory to V. harveyi. Significant difference (p
Not Available
 
Date 2018-11-14T07:01:30Z
2018-11-14T07:01:30Z
2013-05-29
 
Type Research Paper
 
Identifier Not Available
1684-5315
http://krishi.icar.gov.in/Publication/handle/123456789/11127
 
Language English
 
Relation Not Available;
 
Publisher CIBA