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Antagonism of marine macro alga Kappaphycus alvarezii extract against luminescence disease causing Vibrio harveyi during Penaeus monodon larviculture

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Title Antagonism of marine macro alga Kappaphycus alvarezii extract against luminescence disease causing Vibrio harveyi during Penaeus monodon larviculture
Not Available
 
Creator Krishnamoorthy Sivakumar
S. Kannappan
M. Dineshkumar
P.K. Patil
 
Subject Kappaphycus alvarezii extract, antagonism, Vibrio harveyi, virulence factors, shrimp postlarvae, mortality reduction.
 
Description Not Available
Bio-luminescence disease is caused by Vibrio harveyi, it is one of the major diseases in shrimp
hatcheries and grow-out practices. V. harveyi produces virulence factors responsible for pathogenicity.
The use of antibiotics as therapy for luminescence causes development of antibiotic resistance among
the bacterial strains. Henceforth, it is indispensable to use bio-inhibitory agents as substitute for
antibiotics from marine resources. Hence, in this study, marine macro alga Kappaphycus alvarezii was
collected, shadow-dried, pulverized and crude compounds were extracted. This algae extract at 300 μg
level gave zone of inhibition (8.6 mm) against V. harveyi through “agar well diffusion assay”. The 300 g
treated with V. harveyi in LB broth and virulence factors produced were monitored for five days. Results
show that in the control, higher level of lipolysis, thermonuclease, proteolysis, phospholipase were
observed, in the treatment, moderate level was observed. The production of luminescence was reduced
to 6.0, 7.0, 9.7 and 16.0 counts per second (CPS) observed by luminometer for 4 days when compared
with the control (39.6, 50.3, 59.3 and 63.6 CPS). Further, the algae extract at 200 μg/ml was tested during
larviculture for 30 days. The extract gave 29.70% reduction in the cumulative percentage of mortality.
This extract was characterized by FTIR, GC-MS and detected various functional compounds like
alcohols, phenols, alkenes, esters and ethers, etc. These compounds may be responsible for
antagonism against V. harveyi and its virulence factors. This study confirms that the crude K. alvarezii
extract may be a bio-inhibitory agent to control luminescence disease causing V. harveyi.
Not Available
 
Date 2018-11-27T05:06:08Z
2018-11-27T05:06:08Z
2014-02-05
 
Type Research Paper
 
Identifier Not Available
1996-0808
http://krishi.icar.gov.in/jspui/handle/123456789/13540
 
Language English
 
Relation Not Available;
 
Publisher CIBA