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Evaluation of therapeutic potential of selected antifungal chemicals and drugs against Aphanomyces invadans

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Title Evaluation of therapeutic potential of selected antifungal chemicals and drugs against Aphanomyces invadans
Not Available
 
Creator Anutosh Paria
Atul Krishna Dev
Pravata Kumar Pradhan
Rahul Kumar
Gaurav Rathore
Neeraj Sood
 
Subject Aphanomyces invadans
EUS
Formalin
Potassium permanganate
Hydrogen peroxide
Amphotericin B
Fluconazole
In vitro
 
Description The work was carried out under“All India NetworkProject on Fish Health
Infection withAphanomyces invadansis a serious disease affecting freshwater and brackishwaterfish. Even after50 years of itsfirst occurrence, to date, there is no effective treatment for this disease. To develop an effectivetreatment against this disease, in the current study, we have evaluated the potential of six commonly usedchemicals and drugs with known antifungal properties against different life-stages (zoospore production, ger-mination of zoospores and growth of hyphae) ofA. invadans. The chemicals namely formalin, potassium per-manganate (KMnO4), hydrogen peroxide (H2O2), sodium thiosulphate (Na2S2O3) and drugs including ampho-tericin B andfluconazole were evaluated in a dose-dependent manner (0.1, 1.0, 5, 10 and 100 ppm) underinvitroconditions. Out of the chemicals tested, formalin could completely inhibit zoospore production and sig-nificantly inhibit germination of zoospores and growth of hyphae at 10 ppm, however, complete inhibition ofzoospore germination and growth of hyphae could only be observed at 100 ppm. In the case of H2O2, there wassignificant inhibition of germination of zoospores and growth of hyphae at the highest concentration of 100 ppm,but it could not completely inhibit zoospore production, germination of zoospores and growth of hyphae. KMnO4at the highest tested concentration (100 ppm), completely inhibited zoospore production and significantly in-hibited germination of zoospores and growth of hyphae. Na2S2O3was found to be ineffective against all the threelife-stages ofA. invadanseven at the highest tested concentration of 100 ppm. Out of the two antifungal drugs,fluconazole could reduce the germination of zoospores and growth of hyphae significantly at a dose of 1 ppm. Onthe other hand, in the case of amphotericin B, significant inhibition of germination of zoospores and growth ofhypahe was observed only at 100 ppm. Both drugs completely inhibited zoospore production only at 100 ppm.From thefindings of the present study, it can be inferred that out of the six compounds, formalin andfluconazolehave better anti-oomycete effects underin vitroconditions. The information generated would serve as a basis fordeveloping treatment measures against infection withA. invadansin aquaculture ponds
Indian Council ofAgricultural Research is duly acknowledged
 
Date 2021-07-22T06:13:11Z
2021-07-22T06:13:11Z
2020-06-22
 
Type Research Paper
 
Identifier Paria, A., Dev, A.K., Pradhan, P.K., Kumar, R., Rathore, G. and Sood, N., 2020. Evaluation of therapeutic potential of selected antifungal chemicals and drugs against Aphanomyces invadans. Aquaculture, 529, p.735643.
0044-8486
http://krishi.icar.gov.in/jspui/handle/123456789/49315
 
Language English
 
Relation Not Available;
 
Publisher Elsevier