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A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864)

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Title A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864)
Not Available
 
Creator T R. Swaminathan
V. S. Basheer
A. Gopalakrishnan
Neeraj Sood
P. K. Pradhan.
 
Subject Ornamental fish
Horabagrus brachysoma
Yellow cat fish
Fish cell line
 
Description Not Available
A new epithelial cell line, Horabagrus brachysoma fin (HBF), was established from the caudal fin tissue of yellow catfish, H. brachysoma and characterized. This HBF cell line was maintained in Leibovitz's-15 medium supplemented with 15 % fetal bovine serum (FBS) and subcultured more than 62 times over a period of 20 months. The HBF cell line consists predominantly of epithelial cells and is able to grow at temperatures between 20 and 35 °C with an optimum temperature of 28 °C. The growth rate of these cells increased as the proportion of FBS increased from 5 to 20 % at 28 °C with optimum growth at the concentrations of 15 % FBS. Partial amplification and sequencing of fragments of two mitochondrial genes 16S rRNA and COI confirmed that HBF cell line originated from yellow catfish. The HBF cells showed strong positive reaction to the cytokeratin marker, indicating that it was epithelial in nature. HBF cell line was inoculated with tissue homogenate from juveniles of Sea bass, Lates calcarifer infected with viral nervous necrosis virus (VNNV) and found not susceptible to VNNV. The extracellular products of Vibrio cholerae MTCC 3904 were toxic to the HBF cells. These cells were confirmed for the absence of Mycoplasma sp by PCR.
Not Available
 
Date 2021-07-22T10:28:03Z
2021-07-22T10:28:03Z
2014-10-24
 
Type Research Paper
 
Identifier Swaminathan, T.R., Basheer, V.S., Gopalakrishnan, A. et al. A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864). Cytotechnology 68, 515–523 (2016). https://doi.org/10.1007/s10616-014-9804-2
0920-9069
1573-0778
http://krishi.icar.gov.in/jspui/handle/123456789/49390
 
Language English
 
Relation Not Available;
 
Publisher Springer Netherlands