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Phylogenetic analysis of some Puntius species based on 18S rDNA

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Title Phylogenetic analysis of some Puntius species based on 18S rDNA
Not Available
 
Creator RK Saroniya
Nagpure NS
Saksena DN,
Basdeo Kushwaha,
Ravindra Kumar,
Mahender Singh,
Vishwamitra Singh Baisvar,
Akhilesh Kumar Mishra
 
Subject Phylogenetic
rDNA
nucleotide
tree
sequences
 
Description Not Available
The molecular characterization and phylogenetic analysis have been carried out in four Puntius species using 18S rDNA sequences. The size of the consensus sequences of 18S rDNA ranged between 1807-1817 bp (i.e. 1810 bp in P. chola, 1809 bp in P. conchonius, 1817 bp in P. sophore and 1807 bp in P. ticto). The GC and AT content (%) was 56.96 and 43.04 in P. chola, 55.67 and 44.33 in P. conchonius, 56.69 and 43.31 in P. sophore and 55.06 and 44.94 in P. ticto. The dataset after multiple sequence alignment of these Puntius species contained 1906 nucleotide positions, out of which 1430 were conserved, 467 variable, 205 singleton, and 256 parsimony informative. The inter-specific genetic variation was low. P. chola revealed lowest genetic
distance with P. sophore and same was true between P. conchonius and P. ticto. The evolutionary history was inferred by three methods, namely Maximum Parsimony, Neighbour-Joining and the Maximum Likelihood. The three different phylogenetic approaches resulted mostly in similar tree topologies and the clades were well supported with bootstrap values. P. chola and P. sophore clustered together as sister group and same was true with P. conchonius and P. ticto, which showed their close relationship. The phylogenetic reconstruction of present study was also in agreement with earlier studies based on morphometric, cytogenetic studies, mitochondrial cytochrome b gene and restriction fragment polymorphism analysis.
UGC, New Delhi
 
Date 2021-07-24T05:56:36Z
2021-07-24T05:56:36Z
2016-07
 
Type Research Paper
 
Identifier Saroniya, R.K., N.S. Nagpure, D.N. Saksena, B. Kushwaha, R. Kumar, M. Singh, V.S. Baisvar and A.K. Mishra, 2016. Phylogenetic analysis of some Puntius species based on 18S rDNA. European Journal of Biotechnology and Bioscience 4(7): 42-47.
Online ISSN: 2321-9122
http://krishi.icar.gov.in/jspui/handle/123456789/49695
 
Language English
 
Relation Not Available;
 
Publisher AkiNik Publications, Delhi