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Heat and chemical treatments in adult Cyprinus carpio (Pisces cypriniformes) rapidly produce sterile gonads

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Title Heat and chemical treatments in adult Cyprinus carpio (Pisces cypriniformes) rapidly produce sterile gonads
Not Available
 
Creator Sullip Kumar Majhi
Avinash Rambhau Rasal
Basdeo Kushwaha
Sudhir Raizada
 
Subject Busulfan
Elevated water temperature
Gonad sterilisation
Fish
Common carp
 
Description Not Available
Several options have been proposed for eradication of germ cells (GCs) in mammals such as treatment with cytotoxic drugs, irradiation, cold ischemia and hyperthermic treatment. Some of these methods have been also tried in fish but conditions for complete sterilisation of gonads have not been established. Here, we report the production of sterile adult common carp Cyprinus carpio in 10 weeks by the heat and chemical treatments. The cytotoxic drug busulfan (40 mg/kg) was intraperitoneally injected into the animals at 2-week intervals (5 doses in total), and they were maintained in water at 38 °C between Weeks 1 and 10. The effectiveness of the treatments was assessed using gonadal index, histology, and vasa gene expression. At the end of Week 10, very severe gonadal degeneration was observed in fish treated with the heat–chemical combination, and 100% of male and female fish were devoid of endogenous GCs. The average levels of vasa transcript were 0.01 ± 0.005 and 0.02 ± 0.016 for males and females, respectively. By contrast, high temperature alone caused minor gonadal degeneration and the gene transcript were 0.59 ± 0.131 for male and 0.62 ± 0.13 for female. In Week 20, after the recovery period of 10 weeks at 25 °C, the gonadal germ cell did not recover from the sterile condition in any of the sampled individuals. The change in colouration of gonads was an additional useful index of the degree of gonadal sterility.
Not Available
 
Date 2021-07-24T10:20:23Z
2021-07-24T10:20:23Z
2017-08-01
 
Type Research Paper
 
Identifier Majhi, S.K., Rasal, A.R., Kushwaha, B. and Raizada, S., 2017. Heat and chemical treatments in adult Cyprinus carpio (Pisces cypriniformes) rapidly produce sterile gonads. Animal reproduction science, 183, pp.77-85.
ISSN: 0378-4320
http://krishi.icar.gov.in/jspui/handle/123456789/49870
 
Language English
 
Relation Not Available;
 
Publisher Elsevier