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Biofloc-based enhanced survival of Litopenaeus vannamei upon AHPND-causing Vibrio parahaemolyticus challenge is mediated by reduced expression of its virulence genes and enhanced shrimp resistance.

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Title Biofloc-based enhanced survival of Litopenaeus vannamei upon AHPND-causing Vibrio parahaemolyticus challenge is mediated by reduced expression of its virulence genes and enhanced shrimp resistance.
 
Creator Peter Bossier
 
Subject Aquatic animal health management
 
Description Not Available
The biofloc system is relatively a new aquaculture technology that offers a practical solution to maintain culture water quality by recycling nutrients, in particular nitrogen, from unconsumed feed, faecal materials and metabolic products. There are reports, suggesting that a biofloc system improves the health status and resistance of shrimps against microbial infection, yet the mode of action involved remains unclear. This study aimed to unravel the underlying mechanism behind the protective effect of a biofloc system using Litopenaeus vannamei and AHPND-causing Vibrio parahaemolyticus M0904 strain as host-pathogen model. The results showed that a biofloc system maintained at a C/N ratio of 15, improves the water quality and contributes to the nutrition of cultured animals as bioflocs might serve as an additional protein source. Furthermore, the study demonstrated that the biofloc system enhances the survival of L. vannamei upon challenge with a V. parahaemolyticus AHPND strain. The results indicate that the used biofloc system mediates phenotype switching in V. parahaemolyticus resulting in decreased transcription of flagella-related motility genes (flaA, CheR and fliS), Pir toxin (PirBVP) and AHPND plasmid genes (ORF14) and increased expression of the phenotype switching marker AlkPhoX gene in in vitro conditions. Remarkably this phenotypic status in maintained under in vivo conditions (in the hepatopancreas) reducing virulence. Hence, the used biofloc system, steers phenotype switching contributing the decreased virulence of the V. parahaemolyticus AHPND strain towards shrimp postlarvae.
 
Date 2021-04-29T07:10:38Z
2021-04-29T07:10:38Z
2020-1-1
 
Type Research Paper
 
Identifier Not Available
1664-302X
http://krishi.icar.gov.in/jspui/handle/123456789/46844
 
Language English
 
Publisher Not Available