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Genetic heterogeneity of non-O1 and non-O139 Vibrio cholerae isolates from shrimp aquaculture system: a comparison of RS-, REP- and ERIC-PCR fingerprinting approaches

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Title Genetic heterogeneity of non-O1 and non-O139 Vibrio cholerae isolates from shrimp aquaculture system: a comparison of RS-, REP- and ERIC-PCR fingerprinting approaches
Not Available
 
Creator Rao, B.M.
Surendran, P. K.
 
Subject black tiger shrimp
ERIC-PCR
genetic heterogeneity
non-O1 and non-O139
Penaeus monodon
REP-PCR
RS-PCR
Vibriocholerae
 
Description Not Available
Aims: The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequences.

Methods and Results: Non-O1 and non-O139 V. cholerae isolates were obtained from shrimp pond water, pond sediment, shrimp head and shrimp muscle. RS-PCR yielded fewer bands than REP-PCR and ERIC-PCR. Higher similarity was observed in RS-PCR (75–100%) than in REP-PCR (60–95%) and ERIC-PCR (40–95%).

Conclusions: A 100% similarity between V. cholerae isolates was only noticed in RS-PCR. The choleratoxigenic V. cholerae (non-O1 and non-O139) showed greater genetic similarity with ctx-negative V. cholerae than among ctx-positive V. cholerae.

Significance and Impact of the Study: The greater similarity of ctx-positive V. cholerae with ctx-negative V. cholerae isolates indicates that the ctx-positive strains (non-O1 and non-O139) might have originated from autochthonous V. cholerae in the aquatic niche.
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Date 2021-08-03T10:44:27Z
2021-08-03T10:44:27Z
2010-07
 
Type Research Paper
 
Identifier Rao, B. M. and Surendran, P.K. (2010) Genetic heterogeneity of non-O1 and non-O139 Vibriocholerae islates from shrimp aquaculture system: Acomparison of RS-, REP- and ERIC-PCRfingerprinting approaches, Lett. Appl. Microbiol., 51(1):65-74.
0266-8254
http://krishi.icar.gov.in/jspui/handle/123456789/52708
 
Language English
 
Relation Not Available;
 
Publisher Wiley