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Emergence of carp edema virus (CEV) in cultured ornamental koi carp, Cyprinus carpio koi in India

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Title Emergence of carp edema virus (CEV) in cultured ornamental koi carp, Cyprinus carpio koi in India
Not Available
 
Creator T. R. Swaminathan
Raj Kumar
Arathi Dharmaratnam
V. S. Basheer
Neeraj Sood
P. K. Pradhan
N. K. Sanil
P. Vijayagopal
J. K. Jena
 
Subject Cyprinus carpio koi
carp edema virus
koi sleepy disease
 
Description Not Available
A disease outbreak was reported in adult koi, Cyprinus carpio koi, from a fish farm in Kerala, India, during June 2015. The clinical signs were observed only in recently introduced adult koi, and an existing population of fish did not show any clinical signs or mortality. Microscopic examination of wet mounts from the gills of affected koi revealed minor infestation of Dactylogyrus sp. in a few koi. In bacteriological studies, only opportunistic bacteria were isolated from the gills of affected fish. The histopathological examination of the affected fish revealed necrotic changes in gills and, importantly, virus particles were demonstrated in cytoplasm of gill epithelial cells in transmission electron microscopy. The tissue samples from affected koi were negative for common viruses reported from koi viz. cyprinid herpesvirus 3, spring viraemia of carp virus, koi ranavirus and red sea bream iridovirus in PCR screening. However, gill tissue from affected koi carp was positive for carp edema virus (CEV) in the first step of nested PCR, and sequencing of PCR amplicons confirmed infection with CEV. No cytopathic effect was observed in six fish cell lines following inoculation of filtered tissue homogenate prepared from gills of affected fish. In bioassay, the symptoms could be reproduced by inoculation of naive koi with filtrate from gill tissue homogenate of CEV-positive fish. Subsequently, screening of koi showing clinical signs similar to koi sleepy disease from different locations revealed that CEV infection was widespread. To our knowledge, this is the first report of infection with CEV in koi from India.
Not Available
 
Date 2021-07-23T06:41:00Z
2021-07-23T06:41:00Z
2016-10-31
 
Type Research Paper
 
Identifier Swaminathan T. R, Raj Kumar, Arathi Dharmaratnam, V. S. Basheer, Neeraj Sood, P. K. Pradhan, N. K. Sanil, P. Vijayagopal, and J. K. Jena. 2016. Emergence of carp edema virus (CEV) in cultured ornamental koi carp, Cyprinus carpio koi in India. Journal of general Virology 97(12):3392-3399. https://doi.org/10.1099/jgv.0.000649
0022-1317
1465-2099
http://krishi.icar.gov.in/jspui/handle/123456789/49475
 
Language English
 
Relation Not Available;
 
Publisher Cambridge University Press