Record Details

Evolutionary and taxonomic relationships in loach (Genus: Botia) through molecular characterization in a river of Terai region of West Bengal, India

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Evolutionary and taxonomic relationships in loach (Genus: Botia) through molecular characterization in a river of Terai region of West Bengal, India
Not Available
 
Creator Arpita Dey
Rashmi Verma
Mahender Singh
Sudip Barat
 
Subject Genus Botia
Cytochrome Oxidase subunit I gene
Barcoding, Phylogeny
Terai region of West Bengal
 
Description Not Available
Loaches which are quite near to extinction are high demanding fish species having both ornamental and
edible food value. Their identification is difficult due to morphological variation especially amongst
Botia lohachata and Botia almorhae. Genetic diversity and molecular phylogeny among six loaches
belonging to genus Botia were studied. Cytochrome Oxidase I (COI) gene (655 bp) was amplified
using PCR and sequenced. The pairwise genetic distances among Botia species ranged from 0.004 to
0.200. The interspecies Kimura’s 2- parameter pair-wise distance was highest (0.200) between B.
modesta and B. lohachata and lowest (0.004) for B. almorhae and B. lohachata. The overall
transition/transversion bias is R = 3.084. The phylogenetic tree showed that B. almorhae and B.
lohachata formed a monophyletic group (supported by 100% bootstrap value) and then constituted one
clade with B. kubotai. Other Asian species B. rostrata, B. striata, B. dario B. modesta and B.
macracanthus also contributed to this clade but were distant to native Botia species. It was deduced that
loaches need attention towards conservation due to its endangerment and vulnerability status.
Department of Biotechnology, Government of India, New Delhi
 
Date 2021-07-26T05:56:41Z
2021-07-26T05:56:41Z
2015-09
 
Type Research Paper
 
Identifier Dey, A., Verma, R., Singh, M. and Barat, S., 2015. Evolutionary and taxonomic relationships in loach (Genus: Botia) through molecular characterization in a river of Terai region of West Bengal, India, European Journal of Biotechnology and Bioscience, 3(9):12-17
2321-9122
http://krishi.icar.gov.in/jspui/handle/123456789/50153
 
Language English
 
Relation Not Available;
 
Publisher Gupta Publications