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Antibiotic susceptibility pattern of bacteria isolated from freshwater ornamental fish, guppy showing bacterial disease

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Title Antibiotic susceptibility pattern of bacteria isolated from freshwater ornamental fish, guppy showing bacterial disease
Not Available
 
Creator Prasannan Geetha Preena
Arathi Dharmaratnam
Nithianantham Sundar Raj
Thaliyil Veetil Arun Kumar
Shanmuganathan Arun Raja
Thangaraj Raja Swaminathan
 
Subject Antibiotic resistance
Guppy fish .
Ornamenta lfish
Aeromonas
Pseudomonas
Integron
 
Description In the fields of microbial, plant and animal sciences
Antimicrobial resistance is one of the major threats faced in aquaculture systems. Hence the present study is mainly focused on to determine the antimicrobial susceptibility associated with pathogens derived from diseased freshwater ornamental guppy fishes. Around fifteen isolates were resolved from the infected fishes and subjected to antimicrobial susceptibility testing, phenotypic and genotypic characterization. Disc diffusion method was adopted for checking the antibiotic susceptibility using 17 antibiotic discs belonging to different classes. Dendrogram generated 5 clusters based on the biochemical tests and representative isolates were sequenced and identified as Aeromonas hydrophila, Aeromonas sobria, Pseudomonas putida, Acinetobacter soli and Kurthia gibsonii. The Shannon wiener diversity index of the resolved isolates was found to be 1.395 as determined by Primer-E software. Among the isolates, the majority of them was found to be Pseudomonas putida and exhibited higher antibiotic resistance towards antibiotics of 10 classes including third generation Cephalosporin and others showed the same against antibiotics of at least 5 classes tested. All of the recovered isolates possessed the MAR index of greater than 0.2, indicating the heavier dose of antibiotics in the farm. The detection of plasmid-mediated class I integron in Aeromonas hydrophila, Aeromonas sobria and Acinetobacter soli indicated the possibility of heavier dissemination of antimicrobial resistant genes among the ornamental fishes. Gentamycin and Ciprofloxacin were significantly effective against all the isolates and can be successfully applied in aquaculture. The occurrence of antibiotic resistance reminds the proper surveillance and continuous monitoring programmes in the fish farms and usage of other effective alternatives.
Department of Science and Technology (DST) for the Fellowship under National Postdoctoral Fellowship Scheme with Grant number PDF/2017/000378
 
Date 2021-08-02T07:09:27Z
2021-08-02T07:09:27Z
2019-04-30
 
Type Research Paper
 
Identifier Preena, P.G., Dharmaratnam, A., Raj, N.S. et al. Antibiotic susceptibility pattern of bacteria isolated from freshwater ornamental fish, guppy showing bacterial disease. Biologia 74, 1055–1062 (2019). https://doi.org/10.2478/s11756-019-00261-8
0006-3088
http://krishi.icar.gov.in/jspui/handle/123456789/52030
 
Language English
 
Relation Not Available;
 
Publisher Springers