Record Details

Medium optimization and characterization of cell culture system from Penaeus vannamei for adaptation of white spot syndrome virus (WSSV)

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Medium optimization and characterization of cell culture system from Penaeus vannamei for adaptation of white spot syndrome virus (WSSV)
Not Available
 
Creator S Sivakumar
T Raja Swaminathan
R Anandan
N Kalaimani
 
Subject Shrimp cell culture
hemolymph amino acid
hemolymph biochemical
bacterial cytotoxicity
WSSV cytotoxicity
 
Description Describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery.
The lack of shrimp cell lines and difficulty in establishing shrimp cell culture systems, with an appropriate medium is a major concern in the aquaculture sector. The present study attempts to address this issue by developing an in vitro cell culture system from various tissues (hemocytes, heart, lymphoid tissue, hepatopancreas, gill, eye stalk, and muscle) of Penaeus vannamei (P.vannamei) using commercially available L-15 medium. The cell culture medium was formulated using five different media such as HBSCM-1, HBSCM-2, HBSCM-3, HBSCM-4, and HBSCM-5 containing L-proline and glucose with fetal bovine serum (FBS) supplements. Among the different media used, the HBSCM-5 medium with supplements showed good attachment and proliferation of cells with fibroblast-like, epithelioid, round, and adherent cell morphology in hemocyte culture. The same medium was further screened using different tissues to enhance the cell growth. The hemocytes, heart, and lymphoid tissue cells were passaged five times and maintained up to 20 days. Hepatopancreas and gill cells initially showed good morphological features and survived for more than ten days following subculture cells. Eye stalks and muscle cells perished within five days and did not show any unique morphology. The primary hemocyte cells were subjected to species identification, using cytochrome oxidase subunit I (COI) gene. To assess the primary hemocyte cell culture, cells were used for in vitro propagation of white spot syndrome virus (WSSV) and confirmed by the conventional polymerase chain reaction (PCR). Similarly, the primary cells were treated with bacterial extracellular products (ECPs) from Vibrio parahaemolyticus and Vibrio harveyi, to evaluate the cytotoxicity.
Not Available
 
Date 2021-08-02T07:22:22Z
2021-08-02T07:22:22Z
2019-04-19
 
Type Research Paper
 
Identifier S. Sivakumar, T. Raja Swaminathan, R. Anandan, N. Kalaimani, Medium optimization and characterization of cell culture system from Penaeus vannamei for adaptation of white spot syndrome virus (WSSV), Journal of Virological Methods, Volume 270, 2019, Pages 38-45, ISSN 0166-0934, https://doi.org/10.1016/j.jviromet.2019.04.016
0166-0934
http://krishi.icar.gov.in/jspui/handle/123456789/52046
 
Language English
 
Relation Not Available;
 
Publisher Elsevier