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Indirect-ELISA technique for the detection of the red rot pathogen m sugarcane (Sacchaarum spp hybrid) and resistance screening

Indian Agricultural Research Journals

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Title Indirect-ELISA technique for the detection of the red rot pathogen m sugarcane (Sacchaarum spp hybrid) and resistance screening
 
Creator VISWANATHAN, R
PADMANABAN, P
MOHANRAJ, D
JOTHI, R
 
Subject sugarcane, red rot pathogen, ELISA, detection
 
Description  A study was conductcd during 1995-97 on the possibility of identifying resistance to red rot pathogen Colleiotrichumfaicahum Went in sugarcane based on serological technique. Large number of genotypcs are screcned ror red rot resistancein sugarcane every year. To identify the disease resistance at an early stage a new serological techniquc. enzyme linkedimmunosorbent assay was developed based on antiserum developed against the fungus. Early detection of the pathogen well before the symptom development was possible in this technique. When different tissues in the infectcd sugarcane plant were screened for antigen titre. root eyes and bud in the nodal region and internodal samples from pith and white spot gave higher values showing more pathogen colonisation. When 20 different sugarcane varieties were subjected to ELISA test after pathogen inoculation. it showed a clear variation in disease resistance among them as in field testing. Further this technique was fOund reliable to screen sugarcane genotypes for red rot resistance at un earlier stage.
 
Publisher The Indian Journal of Agricultural Sciences
 
Contributor —
 
Date 2013-03-14
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/28264
 
Source The Indian Journal of Agricultural Sciences; Vol 70, No 5 (2000)
0019-5022
 
Language eng
 
Relation http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/28264/12844
 
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