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Effect of culture media and tissue extracts in the mantle explant culture of abalone, Haliotis varia Linnaeus

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Title Effect of culture media and tissue extracts in the mantle explant culture of abalone, Haliotis varia Linnaeus
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Creator Dharmaraj,S
Sukumaran,N
Suja,C P
 
Subject Effect of culture media and tissue extracts in the mantle explant culture of abalone, Haliotis varia Linnaeus
 
Description Not Available
The study is aimed at developing appropriate media for the mantle explant culture of abalone Haliotis varia. The effect of different media viz., L-15, Ham's F12, M199 was studied in combination with 10% Fetal Calf Serum (FCS) and 10% tissue extracts of gonad, mantle and whole body of abalone, H. varia to understand cell behaviour, cell yield and cell adherence in mantle explant culture of H. varia. Cultures with L-15 media gave better cell yield and M199 promoted better cell adherence. Addition of mantle extract to all media enhanced the cell yield to a maximum followed by whole body extract. Addition of whole body extract facilitated in cell adherence followed by the addition of mantle extract.
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Date 2021-09-08T08:20:54Z
2021-09-08T08:20:54Z
2007
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/61810
 
Language English
 
Relation Not Available
 
Publisher Elsevier