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Cellular and molecular immune response and production performance of Indian white shrimp Penaeus indicus (H. Milne-Edwards, 1837), reared in a biofloc-based system with different protein levels of feed

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Title Cellular and molecular immune response and production performance of Indian white shrimp Penaeus indicus (H. Milne-Edwards, 1837), reared in a biofloc-based system with different protein levels of feed
Not Available
 
Creator Akshaya Panigrahi
Rashmi Ranjan Das
Mani Sundaram
Sivakumar
Jannathulla R.
Lalramchhani C.
Jose Antony
Shyne Anand P.S.
Vinay Kumar K
Jayanthi M
Syama Dayal J
 
Subject Penaeus indicus
Biofloc
FCR
FER
Immunomodulation
Phenoloxidase
Lysozyme
Superoxide dismutase
 
Description Not Available
The present study focuses on the immunity and growth of Penaeus indicus fed with varying protein levels (25%,
30%, and 35%) in a biofloc based rearing system. A 120 days growth trial was carried out using juvenile Penaeus
indicus (0.71 ± 0.01) with dietary protein level, 25% (LP), 30% (MP), and 35% (HP), and a control diet-fed with
35% acted as control group resulting in 4 treatments each with four replicates and were randomly assigned 16
tank units (7500 L each). A combination of different carbon sources (molasses, wheat flour, and rice bran in 2:1:1
ratio), yeast and a probiotic (Bacillus sp.) consortium were used for the development of biofloc. At the end of the
trial, the growth parameters of shrimps viz., initial weight, feed conversion ratio (FCR), and daily growth coefficient
(DGC) were computed. The results indicated that shrimp fed with medium (30%) protein (MP) diet
recorded significantly (P < 0.05) improved growth performance compared to high protein fed group (35%) and
low protein (25%) fed group (LP) in a biofloc system and control group (35%). The immunological parameters
such as hemagglutination activity (HA) assay, serum protein, lysozyme, phenol oxidase (PO), and inhibition of
superoxide dismutase (SOD) activity were observed in serum, plasma, and hemocyte lysate supernatant (HLS).
The HA activity, PO activity in plasma was found to be higher in high protein fed animals, whereas medium
protein resulted in enhanced PO activity in serum. Similarly, lysozyme and SOD were inhibited well in high
protein fed animals compared to the low protein fed group. The vital immune genes’s mRNA profiling showed a
potential rise in the expressional pattern in MP and HP treatments compared to LP and control. BGBP (beta-1,3-
glucan binding protein) and hemocyanin mRNA transcript levels were highly upregulated in the HP (5 fold) and
moderately expressed in MP (2 fold) and LP (1–2 fold). The transcripts of peroxinectin, antimicrobial peptides
like crustin showed significant upregulation in HP followed by in MP and LP and control. Likewise, other immune
genes, such as SOD, prophenoloxidase (proPO), showed a similar trend in a marginal way, indicating
immunomodulation in the biofloc groups. This study suggested that biofloc with high protein (35%) supplementation
can substantially enhance the immune response of shrimps, although medium protein level (30%) is
optimum for improving the survival, growth, and in turn economic return in Indian white shrimp.
Not Available
 
Date 2022-04-25T10:42:42Z
2022-04-25T10:42:42Z
2021-09-03
 
Type Journal
 
Identifier Panigrahi, A., Das, R.R., Sundaram, M., Sivakumar, M.R., Jannathulla, R., Lalramchhani, C., Antony, J., Anand, P.S., Kumar, K.V., Jayanthi, M. and Dayal, J.S., 2021. Cellular and molecular immune response and production performance of Indian white shrimp Penaeus indicus (H. Milne-Edwards, 1837), reared in a biofloc-based system with different protein levels of feed. Fish & Shellfish Immunology, 119, pp.31-41.
1050-4648
http://krishi.icar.gov.in/jspui/handle/123456789/71693
 
Language English
 
Relation Not Available;
 
Publisher Elsevier Ltd.