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Molecular characterization, ontogeny and expression profiling of mitochondrial antiviral signaling adapter, MAVS from Asian seabass Lates calcarifer, Bloch (1790)

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Title Molecular characterization, ontogeny and expression profiling of mitochondrial antiviral signaling adapter, MAVS from Asian seabass Lates calcarifer, Bloch (1790)
Not Available
 
Creator Krishnan R, Gireesh Babu P., Jeena K., Tripathi G., Pani Prasad, K*
 
Subject Molecular characterization, ontogeny
 
Description Not Available
Mitochondrial antiviral signaling protein (MAVS), an innate immune signaling adapter coordinates the
signals received from two independent cytosolic pathogen recognition receptors (RIG-1 and MDA5) to
induce antiviral genes. In the present study the MAVS gene of Lates calcarifer (LcMAVS) was cloned and
characterized. The complete cDNA sequence of LcMAVS was 3160 bp and encodes a poly peptide of 577
amino acids. Structural analysis of LcMAVS revealed an N-terminal CARD-like domain, central prolinerich
domain and a C-terminal transmembrane domain. Phylogenetic analysis indicated that LcMAVS
exhibited the closest relationship to P. olivaceous MAVS. LcMAVS was ubiquitously expressed in all tested
tissues of healthy fish viz., brain, gill, heart, liver, spleen, kidney and intestine, with highest transcript
level in spleen. The mRNA transcript level of LcMAVS in different developmental stages showed
constitutive expression in all the stages tested suggesting the maternal transfer of the gene. Significant
up regulation in MAVS expression was observed post nervous necrosis virus (NNV) challenge in vivo in
all the selected tissues. Further, time course analysis showed that LcMAVS transcripts significantly
increased in the brain and spleen tissues after NNV infection. These findings provide useful information
for further elucidating the function of LcMAVS in antiviral innate immune response against NNV in Asian
seabass.
Not Available
 
Date 2022-06-15T07:34:06Z
2022-06-15T07:34:06Z
2017-10-12
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/72542
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER