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Gene structure and comparative and phylogenetic analyses of Catla catla CYP1A full-length cDNA and its responsiveness to benzo(a)pyrene and copper sulphate at early developmental stages

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Title Gene structure and comparative and phylogenetic analyses of Catla catla CYP1A full-length cDNA and its responsiveness to benzo(a)pyrene and copper sulphate at early developmental stages
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Creator Kumari K., Gireesh-Babu P., Kumar S. & Gopal Krishna
 
Subject Biomarker . Heavy metal . Pollution . Carp . Fish . Polyaromatic hydrocarbons
 
Description Not Available
In the present study, full-length CYP1A
cDNA from Catla catla (Catla) has been identified,
and its real-time quantitative reverse transcription PCR
(qRT-PCR) expression has been evaluated in different
tissues, developmental stages (0, 3, 6, 12 and 24 h and 5,
7 and 9 days post-fertilization) and copper sulphate and
benzo(a)pyrene (BaP)-treated 5-day post-fertilization
(dpf) larvae (6 to 6.5 mm). Various structural, comparative
and phylogenetic analyses of the deduced amino
acid sequence revealed that the identified gene of Catla
belongs to the CYP1A1 subfamily. Among different
tissues of Catla, the highest CYP1A expression was
observed in the kidney followed by the liver, muscle,
gill, intestine and brain. CYP1A mRNA expression was
detected during all the larval developmental stages,
including the unfertilized egg with the highest expression
on 9 dpf. BaP (3.5 ppb) and copper sulphate (sublethal
dose 0.516 ppm) challenge test for 96 h to Catla
Not Available
 
Date 2022-06-15T07:37:29Z
2022-06-15T07:37:29Z
2017-08-17
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/72543
 
Language English
 
Relation Not Available;
 
Publisher CrossMark