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In silico analysis and expression studies of kisspeptin gene in C. catla

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Title In silico analysis and expression studies of kisspeptin gene in C. catla
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Creator Rather M.A., Bhat I.A., Rathor P.K., Gireesh-Babu P., Chaudhari A., Kumar S.J., Sharma R.
 
Subject Cloning; Kiss2; tissue distribution; Ontogeny; Reproduction Introduction
 
Description Not Available
We report the characterization of kisspeptin gene which is considered to be
essential for successful animal reproduction. The full-length cDNA sequence of kiss2
was 583bp, consisted of 11bp 5'-UTR (untranslated region) and 194bp 3'-UTR
respectively. Open reading frame (ORF) of 378 bp encoding a putative protein of 125
amino acids. The C. catla kiss2 protein was having a molecular weight (MW) of
14.51kDa and isoelectric point (pI) of 8.46. There were 4 serine (Ser), 4 threonine
(Thr), and 2 tyrosine (Tyr) phosphorylation sites and no N-glycosylation sites on the
predicted protein. The amino acids on positions 8, 11, 24, 80 and 114 were detected
to be ligand binding sites. The signal peptide analysis predicted that C. catla kiss2 is
a secretory protein. Kiss2 protein is localized in nuclear region (49.7%) and the
extracellular region (38.3%) of the cell. Analysis of tissue distribution revealed that,
kiss2 transcripts were predominantly expressed in the brain and gonads, with
expression levels in female higher than those of male. Ontogenetic analysis of kiss2
demonstrated that expression level was low during early phase of development
stages and more expression was observed during mature stage. Overall present
results lay a strong basis for understanding the role of kisspeptin in the
neuroendocrine system in teleosts.
Not Available
 
Date 2022-06-15T09:38:18Z
2022-06-15T09:38:18Z
2016-08-11
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/72545
 
Language English
 
Relation Not Available;
 
Publisher Taylor & Francis