In silico analysis and expression studies of kisspeptin gene in C. catla
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Title |
In silico analysis and expression studies of kisspeptin gene in C. catla
Not Available |
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Creator |
Rather M.A., Bhat I.A., Rathor P.K., Gireesh-Babu P., Chaudhari A., Kumar S.J., Sharma R.
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Subject |
Cloning; Kiss2; tissue distribution; Ontogeny; Reproduction Introduction
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Description |
Not Available
We report the characterization of kisspeptin gene which is considered to be essential for successful animal reproduction. The full-length cDNA sequence of kiss2 was 583bp, consisted of 11bp 5'-UTR (untranslated region) and 194bp 3'-UTR respectively. Open reading frame (ORF) of 378 bp encoding a putative protein of 125 amino acids. The C. catla kiss2 protein was having a molecular weight (MW) of 14.51kDa and isoelectric point (pI) of 8.46. There were 4 serine (Ser), 4 threonine (Thr), and 2 tyrosine (Tyr) phosphorylation sites and no N-glycosylation sites on the predicted protein. The amino acids on positions 8, 11, 24, 80 and 114 were detected to be ligand binding sites. The signal peptide analysis predicted that C. catla kiss2 is a secretory protein. Kiss2 protein is localized in nuclear region (49.7%) and the extracellular region (38.3%) of the cell. Analysis of tissue distribution revealed that, kiss2 transcripts were predominantly expressed in the brain and gonads, with expression levels in female higher than those of male. Ontogenetic analysis of kiss2 demonstrated that expression level was low during early phase of development stages and more expression was observed during mature stage. Overall present results lay a strong basis for understanding the role of kisspeptin in the neuroendocrine system in teleosts. Not Available |
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Date |
2022-06-15T09:38:18Z
2022-06-15T09:38:18Z 2016-08-11 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/72545 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Taylor & Francis
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