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Molecular characterization and expression profiling of 17-beta-hydroxysteroid dehydrogenase 2 and spermatogenesis associated protein 2 genes in endangered catfish, Clarias magur (Hamilton, 1822)

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Title Molecular characterization and expression profiling of 17-beta-hydroxysteroid dehydrogenase 2 and spermatogenesis associated protein 2 genes in endangered catfish, Clarias magur (Hamilton, 1822)
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Creator Agarwal, D., Gireesh-Babu, P., Pavan-kumar, A., Koringa, P., Joshi, C.G., Gora, A.H., Bhat, I., Chaudhari, A.
 
Subject Steroid; OvatideTM; spermatogenesis; 17b-HSD2; SPATA2
 
Description Not Available
The 17-beta-hydroxysteroid dehydrogenase 2 (17b-HSD2) enzyme regulates steroid levels by
the inactivation of estrogen and androgens. Spermatogenesis associated protein 2 (SPATA2)
plays a vital role in spermatogenesis in vertebrates including fish. We report cloning and
characterization of full cds of 17b-HSD2 and SPATA2 genes in Clarias magur. The full-length
cDNA sequences of 17b-HSD2 and SPATA2 were 1187 bp (ORF 1125 bp) and 1806 bp (ORF
1524 bp) encoding 375 and 508 amino acids, respectively. Signal peptide analysis revealed
SPATA2 is nonsecretory, while 17b-HSD2 is a secretory protein. Hydropathy profiles showed
both proteins are hydrophilic in nature. Tissue distribution of both the genes revealed high
mRNA level of SPATA2 in all tissues examined indicating its wide range of expression.
17b-HSD2 indicated higher expression in preparatory phase compared to spawning phase in
ovary while it was opposite in case of testis. SPATA2 showed significantly higher expression
in preparatory phase compared to spawning phase in both ovary and testis. Administration
of OvatideTM (GnRH analog) resulted in upregulation of SPATA2 expression at 6 and 16 h
post-injection while 17b-HSD2 showed upregulation only at 6 h post-injection. To the best
of our knowledge, this is a first report on characterization of 17b-HSD2 and SPATA2 fulllength
cDNA in catfish.
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Date 2022-06-15T10:47:51Z
2022-06-15T10:47:51Z
2018-12-12
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/72557
 
Language English
 
Relation Not Available;
 
Publisher Taylor & Francis