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Single-nucleotide polymorphisms linked to body weight revealed in growth selected Macrobrachium rosenbergii

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Title Single-nucleotide polymorphisms linked to body weight revealed in growth selected Macrobrachium rosenbergii
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Creator Haldar, C., Das, S.P., Pillai, B.R., Pavan-Kumar, A., Gireesh-Babu, P., Das, P. and Chaudhari, A
 
Subject Marker association study.Candidate gene approach .Genotyping .Genetic selection . Growth-associated SNPs
 
Description Not Available
Association of type I single nucleotide polymorphic (SNP) markers with quantitative traits can
provide an effective method for detecting genes and functions that are responsible for performance
variation in domesticated species. In order to discover novel polymorphisms in candidate genes that
could be associated with growth, fragments (175 to 668 bp) from 11 housekeeping, regulatory, and
immune response genes of Macrobrachium rosenbergii previously reported to contain 83 SNPs
were amplified from genomic DNA of 23 growth selected (cumulative genetic gain of 18%) and 23
unselected individuals and sequenced by Sanger’s method. A total of 45 SNPs were identified from
eight genes, of which 20 were novel and 18 were found to be growth associated with allele
frequencies > 0.65 in the selected group. Eleven of these were located in exonic regions of which 3
present in crustacean lipocalin (LIPC) and heat shock protein 21 (HSP21) were nonsynonymous.
In silico prediction indicates that 2 of the non-synonymous alleles may result in higher stability of
the proteins. Of the 5 synonymous growth-associated SNPs, 3 present in phosphoenol pyruvate
carboxykinase (PEPCK), cytochrome oxidase 1 (COX1), and HSP70 were a switch to the
preferred codon. Seven SNPs were located in the 3′UTRs of lectin 3 and 4 (LEC3, LEC4) and
anti-lipopolysaccharide factor 1 (ALF1). Only one altered allele was observed at every locus. No
SNPs were found in NaK-ATPase, mitochondrial manganese superoxide dismutase, and
tachylectin genes. This is the first such marker association study being reported for
M. rosenbergii from India and will be of use in selecting future generations.
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Date 2022-06-15T10:48:13Z
2022-06-15T10:48:13Z
2018-12-11
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/72558
 
Language English
 
Relation Not Available;
 
Publisher CrossMark