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Chitosan-eurycomanone nanoformulation acts on steroidogenesis pathway genes to increase the reproduction rate in fish

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Title Chitosan-eurycomanone nanoformulation acts on steroidogenesis pathway genes to increase the reproduction rate in fish
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Creator Bhat, I. A., Ahmad, I., Nazir, M. I., Bhat. R.A.H., Gireesh-Babu, P., Goswami, M., Sundaray, J.K., Sharma R.
 
Subject Eurycoma longifolia Fish mRNA Aromatase 17β-estradiol
 
Description Not Available
The study was undertaken to explore the molecular mechanism of eurycomanone, a major compound of
Eurycoma longifolia plant in increasing the reproductive processes in the male fish model. Chitosan-nanoconjugated
eurycomanone nanoparticles with a significant particle size [130 nm (CED1); 144.1 nm (CED2)] and stable
zeta potentials ( + 49.1 mV and + 30 mV) were synthesized and evaluated against naked eurycomanone (ED1
and ED2). In present study, short-term and long-term experiments were conducted to evaluate the effect of nanoformulation
on expression of endocrine-related genes, circulating hormone concentrations (Follicle stimulating
hormone, FSH; luteinizing hormone, LH; progesterone, testosterone and 17-β estradiol) and reproductive capacity
of male Clarias magur. In short-term experiment, the sampling of tissues was done on hourly basis after
injection of eurycomanone either alone or with chitosan and long-term experiment was carried for 21 days and
in this the injection was repeated after 7 days and 14 days. Treatments CED1 and CED2 showed controlled and
sustained surge of the transcript level of selected genes (except aromatase) and serum hormones (except 17β-
estradiol) compared to ED1 and ED2 groups. The transcript levels of aromatase and serum 17β-estradiol hormone
showed the declining trend in the chitosan conjugated groups. The gonadosomatic index (GSI), reproductive
capacity, intracellular calcium and selenium and cellular structure of testes were improved in CED1
and CED2 groups compared to other treatments. Furthermore, the effect of chitosan conjugated eurycomanone
was evaluated in primary testicular cells and an increase in the mRNA expression level of endocrine-related
genes was detected. This is the first report of the use of chitosan conjugated eurycomanone and present study
elucidates the molecular mechanism of eurycomanone in increasing the reproductive output in animals.
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Date 2022-06-15T10:48:39Z
2022-06-15T10:48:39Z
1001-01-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/72559
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER