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Development and characterization of a new DRCF cell line from Indian wild strain zebrafish Danio rerio (Hamilton 1822).

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Title Development and characterization of a new DRCF cell line from Indian wild strain zebrafish Danio rerio (Hamilton 1822).
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Creator Meena LL, Goswami M, Chaudhari A, Nagpure NS, Gireesh-Babu P, Dubey A, Das DK.
 
Subject Cell line . Zebrafish . DRCF
 
Description Not Available
Danio rerio, zebrafish, has been widely used as a non-mammalian vertebrate model organism in various studies. The present research describes to develop and characterize a new cell line from a wild strain Indian zebrafish native to Brahmaputra River, Assam, India. The new cell line designated as DRCF was developed from the caudal fin of D. rerio. The cell line was successfully subcultured up to 31 passages. Growth studies
revealed that cell growth of DRCF was optimal at 28 °C in L-15 medium supplemented with 20% FBS. Molecular characterization of the DRCF cell line using mitochondrial
genes namely cytochrome oxidase subunit I gene (COI) and 16S rRNA authenticated the true origin of the cell line. The chromosome analysis of the DRCF cell line expressed its 50 diploid chromosome number of D. rerio. The immunocytochemical characterization of
the cell line exhibited its fibroblastic morphology. The expression of the green fluorescent protein (GFP)
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Date 2022-06-16T04:27:55Z
2022-06-16T04:27:55Z
2020-03-11
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/72590
 
Language English
 
Relation Not Available;
 
Publisher Not Available