Computational and biological approach for studying structure-function of inhibin chimeric peptide antibodies in Clarias batrachus.
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Title |
Computational and biological approach for studying structure-function of inhibin chimeric peptide antibodies in Clarias batrachus.
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Creator |
Ahmed I, Bhat I, Jagtap A, Balasinor D, Kumar NS, Chanu TI, Rani B, Dar S, Leya T, Gora A, Saharan N*
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Subject |
Inhibin chimeric peptide Circular dichroism Protein-peptide docking Anti-peptide antibody Activin receptor signalling
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Description |
Not Available
Inhibins are heterodimeric glycoproteins that belong to transforming growth factor-β superfamily. The present study was aimed at structural elucidation of immunogenic inhibin chimeric peptide (ICP) using immunoinfor- matics tools, to develop anti-ICP antibodies and to understand the mechanism of interaction between HPG-axis and anti-ICP antibodies in adult female Clarias batrachus. Homology modelling predicted that the ICP adopts a turn and random coil, which was further confirmed by circular dichroism (CD). Competitive binding ELISA with ICP and different unrelated peptides confirmed that the developed anti-ICP antiserum is highly specific and critical for the direct interaction to the inhibin-α subunit. The anti-ICP antibodies increased the mRNA transcript levels of follicle stimulating hormone receptor (FSHr), luteinizing hormone receptor (LHr), activin receptor type I (Act RI) and activin receptor type II (Act RII). Further, the serum concentrations of follicle stimulating hormone (FSH), 17β-estradiol (E2) and progesterone increased significantly in anti-ICP antisera treated groups compared to control. Modelled inhibin-α and ICP exhibits better interaction with each other through two salt bridge formations. Also, ICP showed better interaction with betaglycan through positive and polar-based interactions. The results of the present study suggest that immunoneutralization of inhibin bioactivity, confirms the phy- siological role of inhibin as a component of ovarian feedback mechanism regulating FSH secretion through augmentation of gonadotropin and activin receptor signalling pathways that could affect the reproductive success in fishes. Not Available |
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Date |
2022-06-18T05:49:29Z
2022-06-18T05:49:29Z 2018-11-10 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/72879 |
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Language |
English
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Relation |
Not Available;
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ELSEVIER
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