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Evaluation of interferon gamma (IFN-γ) of Labeo rohita as an immunomodulator: in vitro expression model

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Title Evaluation of interferon gamma (IFN-γ) of Labeo rohita as an immunomodulator: in vitro expression model
Not Available
 
Creator Bedekar MK, Soman P, Kole S, Anand D, Tripathi G, Makesh M, Rajendran KV
 
Subject IFN-γ . Labeo rohita . Peripheral blood mononuclear cells . Poly I:C
 
Description Not Available
Interferon gamma (IFN-γ) or type II interferon is a cytokine that is critical for innate and
adaptive immunity against viral and some bacterial and protozoal infections. The importance of IFN-γ in the immune system lies in its ability to inhibit viral replication directly
and most importantly from its immunomodulatory effects. Previously, we successfully
co-administered IFN-γ along with GAPDH gene of Edwardsiella tarda as bicistronic
DNA vaccine in Labeo rohita. In order to ascertain the individual role of IFN-γ, the
present study involves cloning and expression of 552-bp IFN-γ open-reading frame
(ORF) of L. rohita in striped snakehead (SSN-1) cell line using eukaryotic expression
vector system (pQE-TriSystem) followed by transfection in peripheral blood lymphocytes (PBMCs) to evaluate its immunomodulatory ability in comparison to polyinosinicpolycytidylic acid (Poly I:C)-treated PBMCs. The 18.7-kDa protein, expressed in the
pQE-IFNγ-transfected SSN-1 cells, reacted with anti-His antibody in Western blot
confirming it to be recombinant IFN-γ, whereas the relative expression of IFN-γ, iNOS,
Mx, and IL-1β genes in PBMCs was quantified at 24 h and 48 h post treatment by
qPCR. The comparative kinetics of all four genes showed significantly (p < 0.05) high
upregulation pattern in both pQE-IFNγ-transfected cell group and Poly I:C-treated cell
group demonstrating recombinant IFN-γ as an equally efficient inducer like Poly I:C.
Thus, our in vitro experiment results highlight the immunomodulatory potential of
recombinant IFN-γ as an analogue to synthetic Poly I:C which warranted future studies
to further explore the potential of recombinant IFN-γ as an effective vaccine adjuvant
against different microbial invasion
Not Available
 
Date 2022-06-18T05:51:12Z
2022-06-18T05:51:12Z
2018-07-31
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/72884
 
Language English
 
Relation Not Available;
 
Publisher CrossMark