Record Details

Dynamics of infection in selected tissues of White Spot Syndrome Virus-infected Litopenaeus vannamei

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Dynamics of infection in selected tissues of White Spot Syndrome Virus-infected Litopenaeus vannamei
Not Available
 
Creator Jeena K*, Krishnan R, Shyam KU, Gireesh Babu P, Lakra WS, Purushothaman CS, Prasad KP
 
Subject WSSV, virus load, standard curve, Real Time PCR, L. vannamei
 
Description Not Available
White spot syndrome virus (WSSV) remains as the most dreaded pathogen of shrimp
aquaculture since its first incidence in China in 1991. WSSV is a double stranded DNA
virus belonging to the genus Whispovirus of family Nimaviridae. It has a wide host range
and because of its high virulence it can cause 100% mortality in a period of 3-10 days. The
target tissues of WSSV are of ectodermal and mesodermal origin, including gills, cuticular epithelium etc. In the present study, tissue level dynamics of white spot syndrome virus infection in P. vannamei was investigated. For this healthy shrimps were challenged with the virus and a time course quantification of virus load in subcuticular epithelium, gills and pleopods was carried out by Real Time PCR against the generated standard curve, which could detect as minimum as 10 copies of virus in tissue samples. In the examined tissues, the viral load increased as time progressed, however, at different degrees. Compared with gill, viral load was higher in the sub-cuticular epithelium followed by the pleopods. The study provides knowledge regarding the infectivity within the early time periods following infection. This baseline information could potentially contribute in the sensitivity determination during development of diagnostic techniques with enhanced sensitivity which can help to manage the disease to a greater extent.
Not Available
 
Date 2022-06-18T06:02:52Z
2022-06-18T06:02:52Z
2018-05-21
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/72917
 
Language English
 
Relation Not Available;
 
Publisher Excellent Publishers