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New set of PCR primers for SYBR green-based qPCR detection of IMNV in India

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Title New set of PCR primers for SYBR green-based qPCR detection of IMNV in India
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Creator Kokkattunivarthil S, Krishnan R, Kezhedath J, Prasad KP
 
Subject IMNV Real-time PCR SYBR green Viral quantitation
 
Description Not Available
Emerging and existing diseases are the major havoc to the shrimp aquaculture industry. Outbreaks of viral epidemics severely hinder the sustainable farming system with significant economic losses worldwide. The Pacific white shrimp, Penaeus vannamei farming was seriously affected by infectious myonecrosis caused by a double-stranded RNA virus, infectious myonecrosis virus (IMNV). Early and rapid diagnostics is the priority on considering the efficient management and prevention measures. So, the present study utilized the accurate, rapid and specific detection capabilities of real-time PCR on SYBR Green platform to diagnose and quantify the viral load from the infected tissues by designing an efficient PCR primer set. The developed PCR could detect the virus at 98% efficiency with 10 viral copy number as the limit of detection. The standard curve analysis and ampli- fication arithmetics have shown that it can even detect even less than 10 copy numbers of virus in a sample. The standard curve of the assay has shown R2 value of 0.98 and slope of −3.3834 without any significant variations
in inter- and intra- assays. The validated PCR primer pairs and developed SYBR green-based real-time PCR is highly specific, equally sensitive and comparatively economic than the existing TaqMan probe-based PCR for detection of IMNV.
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Date 2022-06-18T07:22:27Z
2022-06-18T07:22:27Z
2018-06-21
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/72925
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER