Record Details

Reference gene selection for quantitative real-time RT-PCR normalization in Clarias magur at different larval developmental stages.

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Reference gene selection for quantitative real-time RT-PCR normalization in Clarias magur at different larval developmental stages.
Not Available
 
Creator Mir IN, Srivastava PP, Bhat IA, Muralidhar AP, Gireesh-Babu P, Varghese T, Chanu TI, Jain KK
 
Subject Clarias, fishes
 
Description Not Available
Reference genes employed for normalizing quantitative PCR data are important for the accurate analysis of gene expression. To date, no reference genes have been screened for developmental gene expression studies in Clarias magur. In the present study, three commonly used and constitutively expressed genes viz. beta actin (β-actin), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and elongation factor-alpha 1 (EFa1) were examined for their efficacy as internal control to avoid any variation during qRT-PCR expression analysis at different developmental stages of C. magur. All the selected housekeeping genes showed a variable level of mRNA expression during the developmental stages of C. magur. Using three independent statistical algorithms (delta-CT, BestKeeper and NormFinder), β-actin and GAPDH were identified as the suitable genes at different developmental stages. However, comprehensive gene stability evaluation denoted β-actin to be the most stable gene for carrying any gene expression studies. The present results, recommend β-actin as the optimal housekeeping gene for qRT-PCR analysis during different developmental stages of C. magur.
Not Available
 
Date 2022-06-18T07:32:54Z
2022-06-18T07:32:54Z
2018-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/72941
 
Language English
 
Relation Not Available;
 
Publisher Not Available