Record Details

Rapid detection of fraudulence in seven commercial shrimp products by species-specific PCR assays

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Rapid detection of fraudulence in seven commercial shrimp products by species-specific PCR assays
Not Available
 
Creator Wilwet L, Shakila RJ, Sivaraman B, Nayak BB, Kumar HS, Jaiswar AK, Jeyasekaran G*
 
Subject Species authentication Seafood adulteration Species-specific PCR mt DNA Shrimps
 
Description Not Available
Species-specific PCR assay was developed for the authentication of commercially important seven shrimp spe- cies. Species-specific primers were designed by targeting the mitochondrial 16S RNA and Internal Transcriber Spacer (ITS1) genes. The PCR assays generated the amplicons of 276, 204, 362, 778, 212, 318 and 330 bp length
for Penaeus vannamei, Fenneopenaeus indicus, Penaeus monodon, Macrobrachium rosenbergii, Metapenaeus affinis, Heterocarpus gibbosus,and Penaeus semisulcatus, respectively. The specificity of primers was confirmed with tar- geted shrimps and non-targeted fishes. The limit of detection of shrimp species was achieved with 1–0.1 ng/μl.
The developed assays were validated with 68 commercial shrimp products, and the results revealed 16% of mislabeling. Since, the developed PCR assays possess specificity, sensitivity, and applicability, it can be used as an official method for authentication in shrimp processing industries by the regulatory authorities.
Not Available
 
Date 2022-06-20T04:45:11Z
2022-06-20T04:45:11Z
2021-01-10
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/73046
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER