ICAR Research Data Repository for Knowledge Management
Silencing and augmentation of IAG hormone transcripts in adult Macrobrachium rosenbergii males affects morphotype transformation.
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View Archive Info| Field | Value | |
| Title |
Silencing and augmentation of IAG hormone transcripts in adult Macrobrachium rosenbergii males affects morphotype transformation.
Not Available |
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| Creator |
Priyadarshi H, Das R, Pavan-Kumar A, Gireesh-Babu P, Javed H, Kumar S, Marappan M, Somdutt KG, Chaudhari A*
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| Subject |
Insulin-like androgenic gland hormone, Heterogeneous individual growth, Lh-RNA, Knockdown, Augmentation, Morphotype, Freshwater prawn
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| Description |
Not Available
Morphotypic differentiation is the external manifestation of dominance hierarchy in Macrobrachium rosenbergii. The intermediate morphotype orange claw (OC) male exhibits the highest growth rate and is subordinate in hierarchy to blue claw (BC) male while dominant on small male (SM). The present study was undertaken to examine the specific role of insulin-like androgenic gland (iag) hormone in morphotype differentiation of M. rosenbergii. To achieve this, RNAi mediated knockdown as well as augmentation of iag transcripts were effected in ∼60 g OC males using plasmid-based constructs pcD-IAGlh and pcD-IAGorf, respectively. The treatments were administered to animals maintained in isolation as well as in community. The knockdown plasmid construct that expresses iag-specific long hairpin RNA caused 16-fold reduction of iag transcripts in the SSN1 cell line in vitro. When injected into OC males living in a community, 2.3-fold iag knockdown was recorded, while in isolated OC males it was 4.2-fold initially, but returned to normal subsequently. Compared with the respective controls, OC to BC transformations in the iag silenced animals were significantly lower in the community-reared group, while no difference was observed in the isolated animals. It is reported here for the first time that iag augmentation in OC males resulted in significantly higher OC to BC transformations, when animals were reared in community. This plasmid-based IAG knockdown approach could be developed into a low stress, feed or immersion treatment for controlling heterogeneous individual growth of M. rosenbergii males in aquaculture. Not Available |
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| Date |
2022-06-20T08:27:54Z
2022-06-20T08:27:54Z 2017-08-21 |
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| Type |
Research Paper
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| Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/73202 |
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| Language |
English
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| Relation |
Not Available;
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| Publisher |
The company of Biologists
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