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Development and partial characterization of primary cell culture from liver tissue of Pangasianodon hypophthalmus.

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Title Development and partial characterization of primary cell culture from liver tissue of Pangasianodon hypophthalmus.
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Creator Vimal Bhartendu, Tripathi Gayatri, Pani Prasad K and Bedekar Megha K
 
Subject Pangasionodon hypophthalmus, hepatocytes, cell culture, partial characterization, mt 16s rRNA
 
Description Not Available
The present study was undertaken to optimize the conditions for development of primary hepatocyte cultures from Pangasionodon hypophthalmus. Liver tissue explants were used as cell seeding source and a protocol for liver cell culture was optimized considering four parameters i.e. incubation temperature, culture media, pH of the media and serum concentration. Among growth media, L-15, Hepatocyte Basal Medium, DMEM and RPMI-1640 with varying concentration of Foetal Bovine Serum (FBS) were tested for their ability to support the growth of cells. Later, the effect on cell confluence at different pH and at different incubation temperature was monitored. The cells were primarily characterized by Best’s carmine staining solution, which was later partially characterized by amplification of mt 16S rRNA gene fragment of 546 bp size that confirmed its origin from the liver explants of P. hypophthalmus at 20th passage. This study proved that the L-15 medium with pH 7.6 and 20% of serum concentration at
incubation temperature of 28 0C yielded the best result in terms of attachment and proliferation of cells which can be used to develop the liver cell line or as a screening tool for further cytological studies.
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Date 2022-06-16T06:32:27Z
2022-06-16T06:32:27Z
2020-02-11
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/72631
 
Language English
 
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Publisher Not Available