Incidence of norovirus in tropical seafood harbouring fecal indicator bacteria.
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Title |
Incidence of norovirus in tropical seafood harbouring fecal indicator bacteria.
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Creator |
Das O, Lekshmi M, Kumar S, Nayak BB*
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Subject |
Norovirus Seafood Shellfish RT-PCR Fecal indicator
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Description |
Not Available
The occurrence of human norovirus (HuNoV) in fresh seafood was studied by reverse transcription-polymerase chain reaction (RT-PCR). Samples (n = 104) comprising of oysters, clams, shrimps and finfish were collected from 3 retail markets, a landing center and a rocky intertidal zone. The samples were subjected to virus con- centration, followed by RNA extraction and RT-PCR. Of these, 43 (41.34%) samples were positive for HuNoV Group II (GII). The fecal contamination status of the samples was determined by the Most Probable Number (MPN) method. All (100%) the samples were fecally contaminated, with the fecal coliform counts ranging from 10 to > 1400 MPN/100 g. The study shows that fresh seafood, especially the bivalve mollusks, can act as ve- hicles for transmission and spread of HuNoV in the community. The high level of HuNoV contamination of seafood not only constitutes a health risk to the domestic consumers, but also compromises the microbiological qualities of seafood meant for international trade. Not Available |
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Date |
2022-06-17T05:08:27Z
2022-06-17T05:08:27Z 2019-11-21 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/72736 |
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Language |
English
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Relation |
Not Available;
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Publisher |
ELSEVIER
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