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Developing markers for Sigatoka leaf spot disease(MycosphaerellamusicolaLeach) resistance in banana(Musa spp.)

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Title Developing markers for Sigatoka leaf spot disease(MycosphaerellamusicolaLeach) resistance in banana(Musa spp.)
 
Creator Nwauzoma, Akagbuo Barth, Uma S., Saraswathi M. S. and Mustaffa M.M .
 
Subject Marker-assisted selection, disease resistance, Musa, random amplification of polymorphic DNA (RAPD), genetic improvement, SCAR.
 
Description Banana and plantain (Musa spp. Linn) are important
staple and cash crops that grow in the humid regions of
Africa, America and Asia (Robinson, 1996). Worldwide
annual production is estimated to be 400 million tons (Till
et al., 2010). Approximately, 90% of the total production
serves as food for domestic consumption. The impact of
diseases and pests, especially Sigatoka leaf spots has
been recognized as a serious constraint to Musa
*Corresponding author. E-mail: drnwabarth@yahoo.com.
production in different parts of the world (Blomme et al.,
2011). In particular, Sigatoka leaf spot (Mycosphaerella
musicola Leach) has become prevalent especially in
areas where black Sigatoka (Mycosphaerella fijiensis
Morelet) is absent and this limits efficient production.
Breeding for resistance has been credited as the most
appropriate and effective method to control Musa
diseases.
Sigatoka leaf spot (Mycosphaerella musicola Leach) disease is a limiting factor in banana production in
India and other places. Breeding for resistance is the most effective method to control Musa diseases.
However, Musa improvement using conventional methods has been hampered due to lack of genetic
variability, resulting to biotechnological approaches. In this regard, marker-assisted selection has
become a reliable method to improve disease resistance in Musa. The objective of this study was to
identify markers that may be linked to Sigatoka leaf spot disease in Musa, using RAPDs and converting
such into sequence characterized amplified region (SCAR). Consequently, a total of 102 oligonucleotide
OPERON primer pairs were used to screen genomic DNA from two resistant cultivars: Calcutta 4 (Musa
acuminate, AA) and Manoranjitham (AAA), and two susceptible cultivars Anaikomban (AA) and Grande
Naine (AAA) with only 11 (10.8%) of the primers being polymorphic. Eventually, OPK 01 and OPK 11
primers in Calcutta 4 were eluted, but only OPK 11 was sequenced and cloned using pGEM-2T vector,
resulting to a band size of 4.3 KB, and the development of two SCAR markers. A FASTA search in the
Musa genome database could not identify corresponding gene sequences that show homology with the
sequenced PCR fragment. Finally, the SCAR marker was used to amplify genomic DNA from the
segregating population which could not discriminate between resistant and susceptible samples. This
may be due to amplification conditions, limited number of primers and most importantly, the absence of
tight linkage with the gene of interest. In conclusion, it may be necessary to screen the segregating
population with more reliable and reproducible amplified fragment length polymorphism.
 
Date 2017-01-19T13:03:56Z
2017-01-19T13:03:56Z
2011-07-06
 
Type Research Paper
 
Identifier 1684–5315
http://krishi.icar.gov.in/jspui/handle/123456789/1653
 
Language English
 
Publisher African Journals