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Simultaneous detection of episomalbanana streak mysorevirus and banana bunchy top virus using multiplex RT–PCR.

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Title Simultaneous detection of episomalbanana streak mysorevirus and banana bunchy top virus using multiplex RT–PCR.
 
Creator Selvarajan R, M Mary Sheeba and V Balasubramanian.
 
Subject Simultaneous detection, episomal, Banana streak Mysore virus, Banana bunchy top virus, multiplex RT-PCR,banana
 
Description Banana and plantains are grown both as a
staple food and major cash crops for
local and export market throughout the
tropical and subtropical areas of the
world, including India. Many viruses are
known to infect banana and plantain, and
cause severe damage to the plant. Among
banana viruses, Banana bunchy top virus
(BBTV) and Banana streak virus (BSV)
are two important DNA viruses that
cause significant economic loss to
banana growers. These viruses are primarily
transmitted through infected
planting material. BBTV and Banana
streak Mysore virus (BSMysV) are
transmitted by banana black aphid, Pentalonia
nigronervosa and mealy bug,
Planococcus citri respectively, in a persistent
manner. BBTV infection can
result into 100% yield loss1
. Significant
yield reduction ranging from 7% to 90%
has been reported due to BSV2,3
The article discusses the study which uses Multiplex PCR-based detection technique to detect banana viruses like Banana bunchy top virus (BBTV) and Banana streak virus (BSV) to prevent further losses due to infestation in India. Researchers extract samples from infected plants and healthy plants using Spectrum Plant total RNA kit to determine existing infection and mixed infection. The study proves that mRT-PCR can extend to detect further RNA viruses of the banana in a single assay.
 
Date 2017-01-21T06:42:55Z
2017-01-21T06:42:55Z
2011-01-01
 
Type Research Paper
 
Identifier 6
http://krishi.icar.gov.in/jspui/handle/123456789/1772
 
Language English
 
Publisher Current Science