Simultaneous detection of episomalbanana streak mysorevirus and banana bunchy top virus using multiplex RT–PCR.
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Simultaneous detection of episomalbanana streak mysorevirus and banana bunchy top virus using multiplex RT–PCR.
|
|
Creator |
Selvarajan R, M Mary Sheeba and V Balasubramanian.
|
|
Subject |
Simultaneous detection, episomal, Banana streak Mysore virus, Banana bunchy top virus, multiplex RT-PCR,banana
|
|
Description |
Banana and plantains are grown both as a staple food and major cash crops for local and export market throughout the tropical and subtropical areas of the world, including India. Many viruses are known to infect banana and plantain, and cause severe damage to the plant. Among banana viruses, Banana bunchy top virus (BBTV) and Banana streak virus (BSV) are two important DNA viruses that cause significant economic loss to banana growers. These viruses are primarily transmitted through infected planting material. BBTV and Banana streak Mysore virus (BSMysV) are transmitted by banana black aphid, Pentalonia nigronervosa and mealy bug, Planococcus citri respectively, in a persistent manner. BBTV infection can result into 100% yield loss1 . Significant yield reduction ranging from 7% to 90% has been reported due to BSV2,3 The article discusses the study which uses Multiplex PCR-based detection technique to detect banana viruses like Banana bunchy top virus (BBTV) and Banana streak virus (BSV) to prevent further losses due to infestation in India. Researchers extract samples from infected plants and healthy plants using Spectrum Plant total RNA kit to determine existing infection and mixed infection. The study proves that mRT-PCR can extend to detect further RNA viruses of the banana in a single assay. |
|
Date |
2017-01-21T06:42:55Z
2017-01-21T06:42:55Z 2011-01-01 |
|
Type |
Research Paper
|
|
Identifier |
6
http://krishi.icar.gov.in/jspui/handle/123456789/1772 |
|
Language |
English
|
|
Publisher |
Current Science
|
|