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Diversity analysis of arrowroot germplasm using ISSR markers

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Title Diversity analysis of arrowroot germplasm using ISSR markers
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Creator Asha, K.I., Krishna Radhika, N., Vineetha, B., Asha Devi, A., Sheela, M.N. and Sreekumar, J.
 
Subject Arrowroot, ISSR, genetic diversity, polymorphism, cluster analysis, molecular characterization
 
Description Not Available
Arrowroot (Maranta arundinacea L.) is an underutilized tuber crop having great potential to be developed
as a carbohydrate source and functional food. Molecular markers have proved to be valuable tools in
the characterization and evaluation of genetic diversity within and between species and populations.
ISSR markers provide more sophisticated analysis of genetic diversity, cultivar identification, population
genetic structure and other events of evolutionary biology processes. The present work is aimed at the
molecular characterization of the seven arrowroot (Maranta arundinacae L.) accessions maintained at
ICAR-Central Tuber Crops Research Institute field genebank using 12 ISSR primers and to estimate the
extent of diversity using the similarity index. DNA isolation was done using DNeasy® Plant Mini Kit
method. Out of the 13 ISSR markers screened, 12 markers gave polymorphic bands in the accessions
screened. The 12 ISSR markers produced a total of 124 bands across the seven samples of which 66
were polymorphic. The phylogenetic tree generated using UPGMA cluster analysis revealed that diversity
exists in M. arundinacea unlike previously reported. The accessions formed two major clusters with two
outliers in the grouping. The Cluster 1 consisted of two accessions i.e. Acc. 1 (M1) and Acc. 6 (M6)
while Cluster 2 consisted of 3 accessions viz. Acc. 2, 4 and 3 (M2, M4 and M3). The two outliers that
got separated were accessions 5 and 7 (M5 and M7). No duplicates were identified in the present
study. The results indicated that the primers selected for the present study will be useful for future
genetic diversity studies and would provide breeders with a genetic base for selection of diverse parents
for crop improvement programmes in arrowroot.
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Date 2017-05-05T09:57:25Z
2017-05-05T09:57:25Z
2015-07-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/3966
 
Language English
 
Relation Not Available;
 
Publisher Not Available