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Optimization and validation of a residue analysis method for glyphosate, glufosinate, and their metabolites in plant matrixes by Liquid Chromatography with Tandem Mass Spectrometry.

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Title Optimization and validation of a residue analysis method for glyphosate, glufosinate, and their metabolites in plant matrixes by Liquid Chromatography with Tandem Mass Spectrometry.
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Creator Dasharath P. Oulkar, Sandip Hingmire, Arnab Goon, Manjusha Jadhav, Bharat Ugare, Ahammed Shabeer Thekkumpurath, and Kaushik Banerjee.
 
Subject Glyphosate, Glufosinate, plant matrices, LC-MS/MS
 
Description Not Available
A sensitive and accurate LC with tandem MS (MS/MS)-based method was developed and validated for the analysis of the herbicide glyphosate, its metabolite aminomethylphosphonic acid (AMPA), and glufosinate after derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) in various plant matrixes. The method also covers direct analysis of the glufosinate metabolites 3-methylphosphinicopropionic acid (3-MPPA) and N-acetyl-glufosinate (NAG). The homogenized samples were extracted with 0.1% formic acid in water–dichloromethane (50 + 50). The aqueous layer was derivatized with FMOC-Cl, cleaned through an HLB SPE cartridge, and determined by LC-MS/MS. The sample size, extraction solvent, sample-to-solvent ratio, derivatization conditions, and cleanup procedure were thoroughly optimized, the LOQs of glyphosate, glufosinate, and AMPA were 0.5 ng/g in grape, corn (leaf and seed), and cotton (leaf, seed, and oil) and 2 ng/g in soybean and tea. The LOQs of NAG and 3-MPPA were 50 ng/g in all the test matrixes, except tea and soybean, for which the LOQ was 100 ng/g. In all cases, average recoveries were >80%. The method successfully performed the estimation of glyphosate in incurred corn and cotton leaf samples collected from supervised field trials.
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Date 2017-06-02T11:04:12Z
2017-06-02T11:04:12Z
2017-01-01
 
Type Research Paper
 
Identifier Dasharath P. Oulkar, Sandip Hingmire, Arnab Goon, Manjusha Jadhav, Bharat Ugare, Ahammed Shabeer Thekkumpurath, and Kaushik Banerjee. 2017. Optimization and validation of a residue analysis method for glyphosate, glufosinate, and their metabolites in plant matrixes by Liquid Chromatography with Tandem Mass Spectrometry. Journal of AOAC International, 100 (3): 1-9.
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http://krishi.icar.gov.in/jspui/handle/123456789/4245
 
Language English
 
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