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Relative Expression of Apocarotenoid Biosynthetic Genes in Developing Stigmas of Crocus sativus L.

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Title Relative Expression of Apocarotenoid Biosynthetic Genes in Developing Stigmas of Crocus sativus L.
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Creator Javid Iqbal Mir, Nazeer Ahmed,Tassem Ahmad Mokhdomi, Asrar Hussain Wafai, Sajad Hassan Wani, Shoiab Bukhari, Asif Amin, Raies Ahmad Qadri1
 
Subject Crocus sativus, CsBCH, CsGT-2, CsLYC, CsZCD, stigma development
 
Description Not Available
Saffron, the desiccated stigmas of Crocus sativus, is recognized for its attractive color, flavor, and aroma which are due to the accumulation of crocin, picrocrocin, and safranal, respectively. HPLC analysis demonstrated maximum apocarotenoid accumulation during the fully developed scarlet stage of stigma development followed by the orange and yellow stages of stigma development. Reverse Transcription-PCR analysis revealed a concurrent expression pattern of CsZCD and CsLYC genes in a developmental stagespecific manner. However, CsBCH and CsGT2 genes were specifically expressed during the mature, scarlet stage of stigma development. Real-Time PCR analysis showed a sharp increase in gene expression of CsLYC gene during stigma development indicative of its possible regulatory role in apocarotenoid biosynthesis or stigma development. Results suggest that genetic manipulation of this gene can help to improve the quality of stigma in saffron; besides highlighting its potential to monitor stigma development during in vitro experimentation.
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Date 2018-10-22T05:45:22Z
2018-10-22T05:45:22Z
2013-09-01
 
Type Research Paper
 
Identifier J. Crop Sci. Biotech. 2013 (Sep) 16 (3) : 183~188
http://krishi.icar.gov.in/jspui/handle/123456789/8034
 
Language English
 
Relation Not Available;
 
Publisher Korean Society of Crop Science and Springer 2013